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Title

Spin densities in flavin analogs within a flavoprotein

AuthorsMartínez, Jesús I.; Alonso, Pablo J.; García-Rubio, Inés; Medina, Milagros
Issue Date2016
PublisherBiophysical Society
Elsevier
CitationBiophysical Journal 110(3): 561-571 (2016)
AbstractCharacterization by electron paramagnetic resonance techniques of several variants of Anabaena flavodoxin, where the naturally occurring FMN cofactor is substituted by different analogs, makes it possible to improve the details of the spin distribution map in the isoallosazine ring in its semiquinone state. The analyzed variants were selected to monitor the effects of intrinsic changes in the flavin ring electronic structure, as well as perturbations in the apoflavodoxin-flavin interaction, on the spin populations. When these effects were analyzed together with the functional properties of the different flavodoxin variants, a relationship between spin population and biochemical parameters, as the reduction potential, could be envisaged.
URIhttp://hdl.handle.net/10261/148211
DOI10.1016/j.bpj.2015.11.3525
Identifiersdoi: 10.1016/j.bpj.2015.11.3525
issn: 0006-3495
e-issn: 1542-0086
Appears in Collections:(ICMA) Artículos
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