English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/148144
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Combined miRNA profiling and proteomics demonstrates that different miRNAs target a common set of proteins to promote colorectal cancer metastasis

Other TitlesmiRNAs in colorectal cancer metastasis
AuthorsTorres, Sofía ; García-Palmero, Irene ; Bartolomé, Rubén Álvaro ; Fernandez-Aceñero, M. Jesús; Molina, Elena ; Calviño, Eva ; Segura, Miguel F.; Casal, J. Ignacio
KeywordsMetastasis
Colorectal cancer
miR-424-3p
miR-503
miR-1292
CKB
UBA2
Issue Date7-Mar-2017
PublisherJohn Wiley & Sons
CitationThe Journal of Pathology (2017)
AbstractThe process of liver colonization in colorectal cancer remains poorly characterized. Here, we addressed the role of microRNA (miRNA) dysregulation in metastasis. We first compared miRNA expression profiles between colorectal cancer cell lines with different metastatic properties and then identified target proteins of the dysregulated miRNAs to establish their functions and prognostic value. We found that 38 miRNAs were differentially expressed between highly metastatic (KM12SM/SW620) and poorly metastatic (KM12C/SW480) cancer cell lines. After initial validation, we determined that three miRNAs (miR-424-3p, −503, and −1292) were overexpressed in metastatic colorectal cancer cell lines and human samples. Stable transduction of non-metastatic cells with each of the three miRNAs promoted metastatic properties in culture and increased liver colonization in vivo. Moreover, miR-424-3p and miR-1292 were associated with poor prognosis in human patients. A quantitative proteomic analysis of colorectal cancer cells transfected with miR-424-3p, miR-503, or miR-1292 identified alterations in 149, 129, or 121 proteins, respectively, with an extensive overlap of the target proteins of the three miRNAs. Importantly, down-regulation of two of these shared target proteins, CKB and UBA2, increased cell adhesion and proliferation in colorectal cancer cells. The capacity of distinct miRNAs to regulate the same mRNAs boosts the capacity of miRNAs to regulate cancer metastasis and underscores the necessity of targeting multiple miRNAs for effective cancer therapy. Copyright © 2017 Pathological Society of Great Britain and Ireland.
Description39 p.-6 fig.
Publisher version (URL)http://dx.doi.org/10.1002/path.4874
URIhttp://hdl.handle.net/10261/148144
DOI10.1002/path.4874
ISSN0022-3417
E-ISSN1096-9896
Appears in Collections:(CIB) Artículos
Files in This Item:
File Description SizeFormat 
J Pathol.2017pdf.pdfPostprint2,55 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.