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Synthesis of overlapping fibrin citrullinated peptides and their use for diagnosing rheumatoid arthritis

AuthorsPérez Rodríguez, María L.; Gómara Elena, María José ; Kasi, Devi; Alonso, Ana ; Viñas, O.; Ercilla, Guadalupe; Sanmartí, Raimon; Haro Villar, Isabel
Enzyme-linked immunosorbent assay
Rheumatoid arthritis
Synthetic peptides
Issue DateOct-2006
PublisherBlackwell Publishing
CitationChemical Biology and Drug Design 68(4): 194-200 (2006)
AbstractWith the aim of developing a new enzyme-linked immunosorbent assay test to detect autoantibodies in the sera of rheumatoid arthritis patients with a high sensitivity and specificity using synthetic citrullinated peptides of fibrin (which is abundant in rheumatoid synovium) as antigenic substract, peptides belonging to α- and β-fibrin chains were selected by computer-aided prediction of antigenicity and epitope mapping and synthesized in solid phase. We analysed by enzyme-linked immunosorbent assay 133 sera from patients with well-characterized rheumatic diseases, including 67 patients with rheumatoid arthritis. The results of the immunoassays reported highlight the usefulness of fibrin-related peptides in rheumatoid arthritis diagnosis and, especially, the ability and specificity of the [Cit621,627,630]α-fibrin(617–631) (αfib617) peptide sequence to recognize the autoantibodies that are present in rheumatoid arthritis patients.
Description7 pages, 2 figures.-- PMID: 17105483 [PubMed].
Publisher version (URL)http://dx.doi.org/10.1111/j.1747-0285.2006.00438.x
Appears in Collections:(IQAC) Artículos
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