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Title

Determination of allatostatin levels in relation to the gonadotropic cycle in the female of Blattella germanica (L.) (Dictyoptera, Blattellidae)

AuthorsVilaplana, Luisa; Maestro, José L. ; Piulachs, Maria-Dolors ; Bellés, Xavier
KeywordsELISA
Haemolymph
Midgut.
Brain
Allatostatin
Blattella germanica
Issue DateSep-1999
PublisherJohn Wiley & Sons
CitationPhysiological Entomology 24(3): 213-219 (1999)
AbstractA polyclonal antibody against the allatostatin BLAST-3 (AGSDGRLYSFGL-NH2) of the cockroach Blattella germanica (L.) (Dictyoptera, Blattellidae) has been raised and characterized, and an ELISA (enzyme-linked immunosorbent assay) for allatostatin quantification has been developed. Allatostatin contents in brain, midgut and haemolymph have been measured in females of B. germanica during the first gonadotropic cycle. Brain allatostatin content increases steadily from adult emergence to the formation of the first ootheca. The values range from 2 ng/brain on the day of adult emergence to 25 ng/brain when the insect forms the ootheca 8 days later. In the midgut, the pattern is similar but the values are about half those of the brain. Allatostatin concentrations in the haemolymph after HPLC separation are in the nanomolar range. The occurrence of allatostatins in the haemolymph suggests that these peptides can act through a humoral pathway, as well as via nerves. The allatostatin content of both brain and midgut are high while the female is transporting the ootheca, which suggests that these peptides could be related to the low metabolic status characterizing the period of oothecal transport.
Publisher version (URL)http://doi.org/10.1046/j.1365-3032.1999.00132.x
URIhttp://hdl.handle.net/10261/147944
DOI10.1046/j.1365-3032.1999.00132.x
Identifiersissn: 0307-6962
e-issn: 1365-3032)
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