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| Título: | BaitFisher: A software package for multispecies target DNA enrichment probe design |
Autor: | Mayer, Christoph Urs; Bleidorn, Christoph CSIC ORCID | Palabras clave: | Phylogenetics Hymenoptera Comparative genomics Hybrid enrichment Phylogenomics |
Fecha de publicación: | jul-2016 | Editor: | Oxford University Press | Citación: | Molecular Biology and Evolution 33(7): 1875-1886 (2016) | Resumen: | Target DNA enrichment combined with high-throughput sequencing technologies is a powerful approach to probing a large number of loci in genomes of interest. However, software algorithms that explicitly consider nucleotide sequence information of target loci in multiple reference species for optimizing design of target enrichment baits to be applicable across a wide range of species have not been developed. Here we present an algorithm that infers target DNA enrichment baits from multiple nucleotide sequence alignments. By applying clustering methods and the combinatorial 1-center sequence optimization to bait design, we are able to minimize the total number of baits required to efficiently probe target loci in multiple species. Consequently, more loci can be probed across species with a given number of baits. Using transcript sequences of 24 apoid wasps (Hymenoptera: Crabronidae, Sphecidae) from the 1KITE project and the gene models of Nasonia vitripennis, we inferred 57,650, 120-bp-long baits for capturing 378 coding sequence sections of 282 genes in apoid wasps. Illumina reduced-representation library sequencing confirmed successful enrichment of the target DNA when applying these baits to DNA of various apoid wasps. The designed baits furthermore enriched a major fraction of the target DNA in distantly related Hymenoptera, such as Formicidae and Chalcidoidea, highlighting the baits' broad taxonomic applicability. The availability of baits with broad taxonomic applicability is of major interest in numerous disciplines, ranging from phylogenetics to biodiversity monitoring. We implemented our new approach in a software package, called BaitFisher, which is open source and freely available at https://github.com/cmayer/BaitFisher-package.git. | Descripción: | Christoph Urs Mayer [et al.] | URI: | http://hdl.handle.net/10261/147842 | DOI: | 10.1093/molbev/msw056 | Identificadores: | doi: 10.1093/molbev/msw056 issn: 0737-4038 |
| Aparece en las colecciones: | (MNCN) Artículos |
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| Fichero | Descripción | Tamaño | Formato | |
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| MBEvo 33(7) 1875-1886 (2016).pdf | 514,76 kB | Adobe PDF |  Visualizar/Abrir |
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