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dc.contributor.authorMartínez Fernández, Irenees_ES
dc.contributor.authorSanchís, Sofíaes_ES
dc.contributor.authorMarini, Nacielees_ES
dc.contributor.authorBalanza Pérez, Vicentees_ES
dc.contributor.authorBallester, Patriciaes_ES
dc.contributor.authorNavarrete-Gómez, Marisaes_ES
dc.contributor.authorOliveira, Antonio C. dees_ES
dc.contributor.authorColombo, Luciaes_ES
dc.contributor.authorFerrándiz, Cristinaes_ES
dc.identifier.citationFrontiers in Plant Science 5: 210 (2014)es_ES
dc.description.abstractThe four NGATHA genes (NGA) form a small subfamily within the large family of B3-domain transcription factors of Arabidopsis thaliana. NGA genes act redundantly to direct the development of the apical tissues of the gynoecium, the style, and the stigma. Previous studies indicate that NGA genes could exert this function at least partially by directing the synthesis of auxin at the distal end of the developing gynoecium through the upregulation of two different YUCCA genes, which encode flavin monooxygenases involved in auxin biosynthesis. We have compared three developing pistil transcriptome data sets from wildtype, nga quadruple mutants, and a 35S::NGA3 line. The differentially expressed genes showed a significant enrichment for auxin-related genes, supporting the idea of NGA genes as major regulators of auxin accumulation and distribution within the developing gynoecium. We have introduced reporter lines for several of these differentially expressed genes involved in synthesis, transport and response to auxin in NGA gain- and loss-of-function backgrounds. We present here a detailed map of the response of these reporters to NGA misregulation that could help to clarify the role of NGA in auxin-mediated gynoecium morphogenesis. Our data point to a very reduced auxin synthesis in the developing apical gynoecium of nga mutants, likely responsible for the lack of DR5rev::GFP reporter activity observed in these mutants. In addition, NGA altered activity affects the expression of protein kinases that regulate the cellular localization of auxin efflux regulators, and thus likely impact auxin transport. Finally, protein accumulation in pistils of several ARFs was differentially affected by nga mutations or NGA overexpression, suggesting that these accumulation patterns depend not only on auxin distribution but could be also regulated by transcriptional networks involving NGA factors.es_ES
dc.description.sponsorshipThis work was supported by the Spanish Ministerio de Ciencia e Innovacion (grant no. BIO2009–09920 to Cristina Ferrándiz), the Fondazione Cariplo (grant “Fruitalia” to Lucia Colombo and Cristina Ferrándiz), and the European Union (grant no. FP7–PEOPLE–PIRSES–2009–247589 to Lucia Colombo, Cristina Ferrándiz, Antonio C. Oliveira).es_ES
dc.publisherFrontiers Media-
dc.relation.isversionofPublisher's versiones_ES
dc.subjectGynoecium development-
dc.subjectAuxin synthesis-
dc.subjectAuxin transport-
dc.titleThe effect of NGATHA altered activity on auxin signaling pathways within the Arabidopsis gynoeciumes_ES
dc.description.peerreviewedPeer reviewedes_ES
dc.contributor.funderMinisterio de Ciencia e Innovación (España)-
dc.contributor.funderFondazione Cariplo-
dc.contributor.funderEuropean Commission-
oprm.item.hasRevisionno ko 0 false*
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