English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/147770
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

The effect of NGATHA altered activity on auxin signaling pathways within the Arabidopsis gynoecium

AuthorsMartínez Fernández, Irene ; Sanchís, Sofía; Marini, Naciele; Balanza Pérez, Vicente ; Ballester, Patricia ; Navarrete-Gómez, Marisa ; Oliveira, Antonio C. de; Colombo, Lucia; Ferrándiz, Cristina
KeywordsGynoecium development
NGATHA
Auxin synthesis
Auxin transport
AUXINRESPONSEFACTORS
Issue Date21-May-2014
PublisherFrontiers Media
CitationFrontiers in Plant Science 5: 210 (2014)
AbstractThe four NGATHA genes (NGA) form a small subfamily within the large family of B3-domain transcription factors of Arabidopsis thaliana. NGA genes act redundantly to direct the development of the apical tissues of the gynoecium, the style, and the stigma. Previous studies indicate that NGA genes could exert this function at least partially by directing the synthesis of auxin at the distal end of the developing gynoecium through the upregulation of two different YUCCA genes, which encode flavin monooxygenases involved in auxin biosynthesis. We have compared three developing pistil transcriptome data sets from wildtype, nga quadruple mutants, and a 35S::NGA3 line. The differentially expressed genes showed a significant enrichment for auxin-related genes, supporting the idea of NGA genes as major regulators of auxin accumulation and distribution within the developing gynoecium. We have introduced reporter lines for several of these differentially expressed genes involved in synthesis, transport and response to auxin in NGA gain- and loss-of-function backgrounds. We present here a detailed map of the response of these reporters to NGA misregulation that could help to clarify the role of NGA in auxin-mediated gynoecium morphogenesis. Our data point to a very reduced auxin synthesis in the developing apical gynoecium of nga mutants, likely responsible for the lack of DR5rev::GFP reporter activity observed in these mutants. In addition, NGA altered activity affects the expression of protein kinases that regulate the cellular localization of auxin efflux regulators, and thus likely impact auxin transport. Finally, protein accumulation in pistils of several ARFs was differentially affected by nga mutations or NGA overexpression, suggesting that these accumulation patterns depend not only on auxin distribution but could be also regulated by transcriptional networks involving NGA factors.
Publisher version (URL)http://doi.org/10.3389/fpls.2014.00210
URIhttp://hdl.handle.net/10261/147770
DOI10.3389/fpls.2014.00210
ISSN1664-462X
Appears in Collections:(IBMCP) Artículos
Files in This Item:
File Description SizeFormat 
fpls-05-00210.pdfArtículo principal1,14 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.