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Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/147744
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dc.contributor.authorGómez-Torres, María José-
dc.contributor.authorMedina Escudero, Sonia-
dc.contributor.authorGil-Izquierdo, Ángel-
dc.contributor.authorOrduna, Jesús-
dc.contributor.authorSavirón, María-
dc.date.accessioned2017-04-03T07:18:47Z-
dc.date.available2017-04-03T07:18:47Z-
dc.date.issued2015-
dc.identifierdoi: 10.1186/s12958-015-0118-9-
dc.identifiere-issn: 1477-7827-
dc.identifier.citationReproductive Biology and Endocrinology 13: 123 (2015)-
dc.identifier.urihttp://hdl.handle.net/10261/147744-
dc.descriptionThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License.-- et al.-
dc.description.abstract[Background]: Fertilization is a key physiological process for the preservation of the species. Consequently, different mechanisms affecting the sperm and the oocyte have been developed to ensure a successful fertilization. Thus, sperm acrosome reaction is necessary for the egg coat penetration and sperm-oolema fusion. Several molecules are able to induce the sperm acrosome reaction; however, this process should be produced coordinately in time and in the space to allow the success of fertilization between gametes. The goal of this study was to analyze the metabolites secreted by cumulus-oocyte-complex (COC) to find out new components that could contribute to the induction of the human sperm acrosome reaction and other physiological processes at the time of gamete interaction and fertilization. [Methods]: For the metabolomic analysis, eighteen aliquots of medium were used in each group, containing: a) only COC before insemination and after 3 h of incubation; b) COC and capacitated spermatozoa after insemination and incubated for 16–20 hours; c) only capacitated sperm after 16–20 h in culture and d) only fertilization medium as control. Six patients undergoing assisted reproduction whose male partners provided normozoospermic samples were included in the study. Seventy-two COC were inseminated. [Results]: The metabolites identified were monoacylglycerol (MAG), lysophosphatidylcholine (LPC) and phytosphingosine (PHS). Analysis by PCR and in silico of the gene expression strongly suggests that the cumulus cells contribute to the formation of the PHS and LPC. [Conclusions]: LPC and PHS are secreted by cumulus cells during in vitro fertilization and they could be involved in the induction of human acrosome reaction (AR). The identification of new molecules with a paracrine effect on oocytes, cumulus cells and spermatozoa will provide a better understanding of gamete interaction.-
dc.description.sponsorshipThis study was supported by grant GV/2009/097 from Department of Education, Generalitat Valenciana, Vicerrectorado de Investigación, University of Alicante, Alicante, Spain (Vigrob-137), the Spanish Ministerio de Economía y Competitividad AGL2012-40180-C03-02 and Fundación Seneca (04542/GERM/06).-
dc.publisherBioMed Central-
dc.relation.isversionofPublisher's version-
dc.rightsopenAccess-
dc.subjectMetabolomics-
dc.subjectPhytosphingosine-
dc.subjectCumulus cells-
dc.subjectLysophosphatidylcholine-
dc.subjectAcrosome reaction-
dc.titleMetabolites involved in cellular communication among human cumulus-oocyte-complex and sperm during in vitro fertilization-
dc.typeartículo-
dc.identifier.doi10.1186/s12958-015-0118-9-
dc.relation.publisherversionhttps://doi.org/10.1186/s12958-015-0118-9-
dc.date.updated2017-04-03T07:18:48Z-
dc.description.versionPeer Reviewed-
dc.language.rfc3066eng-
dc.rights.licensehttp://creativecommons.org/licenses/by/4.0/-
dc.contributor.funderFundación Séneca-
dc.contributor.funderMinisterio de Economía y Competitividad (España)-
dc.contributor.funderGeneralitat Valenciana-
dc.contributor.funderUniversidad de Alicante-
dc.relation.csicSí-
dc.identifier.funderhttp://dx.doi.org/10.13039/100007801es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003329es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003359es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/100009092es_ES
dc.identifier.pmid26553294-
dc.type.coarhttp://purl.org/coar/resource_type/c_6501es_ES
item.fulltextWith Fulltext-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.grantfulltextopen-
item.openairetypeartículo-
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