Gold nanoparticle-siRNA mediated oncogene knockdown at RNA and protein level, with associated gene effects

Abstract

[Aims]: RNAi is a powerful tool for gene silencing that can be used to reduce undesirable overexpression of oncogenes as a novel form of cancer treatment. However, when using RNAi as a therapeutic tool there is potential for associated gene effects. This study aimed to utilize gold nanoparticles to deliver siRNA into HeLa cells. [Results]: Knockdown of the c-myc oncogene by RNAi, at the RNA, protein and cell proliferation level was achieved, while also identifying associated gene responses. [Discussion]: The gold nanoparticles used in this study present an excellent delivery platform for siRNA, but do note associated gene changes. [Conclusion]: The study highlights the need to more widely assess the cell physiological response to RNAi treatment, rather than focus on the immediate RNA levels.