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Gold nanoparticle-siRNA mediated oncogene knockdown at RNA and protein level, with associated gene effects

AuthorsHernandez, Yulán; Conde, João; Baptista, Pedro V.; Fuente, Jesús M. de la; Berry, Catherine Cecilia
Issue Date2015
PublisherFuture Medicine
CitationNanomedicine 10(16): 2513-2525 (2015)
Abstract[Aims]: RNAi is a powerful tool for gene silencing that can be used to reduce undesirable overexpression of oncogenes as a novel form of cancer treatment. However, when using RNAi as a therapeutic tool there is potential for associated gene effects. This study aimed to utilize gold nanoparticles to deliver siRNA into HeLa cells. [Results]: Knockdown of the c-myc oncogene by RNAi, at the RNA, protein and cell proliferation level was achieved, while also identifying associated gene responses. [Discussion]: The gold nanoparticles used in this study present an excellent delivery platform for siRNA, but do note associated gene changes. [Conclusion]: The study highlights the need to more widely assess the cell physiological response to RNAi treatment, rather than focus on the immediate RNA levels.
Identifiersdoi: 10.2217/nnm.15.95
e-issn: 1748-6963
issn: 1743-5889
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