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dc.contributor.authorMangot, Jean-François-
dc.contributor.authorLogares, Ramiro-
dc.contributor.authorSánchez, Pablo-
dc.contributor.authorLatorre, Fran-
dc.contributor.authorMassana, Ramon-
dc.date.accessioned2017-03-31T06:54:33Z-
dc.date.available2017-03-31T06:54:33Z-
dc.date.issued2017-01-
dc.identifierdoi: 10.1038/srep41498-
dc.identifierissn: 2045-2322-
dc.identifier.citationScientific Reports 7: 41498 (2017)-
dc.identifier.urihttp://hdl.handle.net/10261/147663-
dc.descriptionMangot, Jean-François et al.-- 12 pages, 7 figures, 2 tables, supplementary information https://dx.doi.org/10.1038/srep41498-
dc.description.abstractPico-sized eukaryotes play key roles in the functioning of marine ecosystems, but we still have a limited knowledge on their ecology and evolution. The MAST-4 lineage is of particular interest, since it is widespread in surface oceans, presents ecotypic differentiation and has defied culturing efforts so far. Single cell genomics (SCG) are promising tools to retrieve genomic information from these uncultured organisms. However, SCG are based on whole genome amplification, which normally introduces amplification biases that limit the amount of genomic data retrieved from a single cell. Here, we increase the recovery of genomic information from two MAST-4 lineages by co-assembling short reads from multiple Single Amplified Genomes (SAGs) belonging to evolutionary closely related cells. We found that complementary genomic information is retrieved from different SAGs, generating co-assembly that features >74% of genome recovery, against about 20% when assembled individually. Even though this approach is not aimed at generating high-quality draft genomes, it allows accessing to the genomic information of microbes that would otherwise remain unreachable. Since most of the picoeukaryotes still remain uncultured, our work serves as a proof-of-concept that can be applied to other taxa in order to extract genomic data and address new ecological and evolutionary questions-
dc.description.sponsorshipThis work was supported by the US NSF grants DEB-1031049 and OCE-821374 (to M.E.S.), by the ANR French projects Oceanomics (ANR-11-BTBR-0008, to C.V.), France Génomique (ANR-10-INBS-09, to P.W.), and Prometheus (ANR-09-PCS-GENM_217, to O.J.), by the EU project SINGEK (H2020-MSCA-ITN-2015-675752, to R.M.), and by the Spanish project MEFISTO (CTM2013-43767-P, MINECO). J.-F.M. was supported by a Marie Curie Intra-European Fellowship (PIEF-GA-2012-331190, EU). R.L. was supported by Juan de la Cierva (JCI-2010-06594, MINECO) and Ramón y Cajal fellowships (RYC-2013-12554, MINECO)-
dc.publisherNature Publishing Group-
dc.relationinfo:eu-repo/grantAgreement/EC/H2020/675752-
dc.relationMINECO/ICTI2013-2016/CTM2013-43767-P-
dc.relationMINECO/ICTI2013-2016/RYC-2013-12554-
dc.relation.isversionofPublisher's version-
dc.rightsopenAccess-
dc.titleAccessing the genomic information of unculturable oceanic picoeukaryotes by combining multiple single cells-
dc.typeartículo-
dc.identifier.doi10.1038/srep41498-
dc.relation.publisherversionhttps://dx.doi.org/10.1038/srep41498-
dc.date.updated2017-03-31T06:54:33Z-
dc.description.versionPeer Reviewed-
dc.language.rfc3066eng-
dc.rights.licensehttps://creativecommons.org/licenses/by/4.0/-
dc.contributor.funderMinisterio de Economía y Competitividad (España)-
dc.contributor.funderAgence Nationale de la Recherche (France)-
dc.contributor.funderNational Science Foundation (US)-
dc.contributor.funderEuropean Commission-
dc.contributor.funderFrance Génomique-
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003329es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100001665es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/100000001es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100000780es_ES
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