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The fleshless mutation: a valuable source to investigate the regulation of pericarp morphogenesis

AutorTorregrosa, Laurent; Fernandez, Lucie ; Martínez-Zapater, José M. ; Thomas, Mark R.
Fecha de publicaciónabr-2013
CitaciónIX International Symposium on Grapevine Physiology and Biotechnology (2013)
ResumenThe FLESHLESS BERRY (Flb) somatic variation was identified in the grapevine as a chimeric phenotype that develops small fruits due to minimal flesh (Femandez et al., 2006). The lack of flesh development was shown to result from the impairment ofboth cell division and expansion. Interestingly, the phenotype was found to be inherited as a dominant trait, with occassional phenotypic reversions to the wild-type grapevine berry being observed in the original somatic variant and derived populations. Several experimental approaches were performed to: i) decipher the causal genetic mechanism of the abnorrnal flesh morphogenesis due to the jlb mutation and ii) identify the main genes associated with the fleshless phenotype as poten tia! critical players of early fruit development in the grapevine. Fine mapping using microvine progenies (Chaib et al., 2010) and gene sequencing identified the insertion of an active MITE (Miniature Inverted-repeat Transposable Element) in the promoter of the PISTILLATA-like gene (VvPJ) as the cause of the fleshless phenotype and variations. The TE insertion caused specific ectopic activation of a VvPI allele during early fruit development that maintains the expression of floral genes within the fruit. The analysis of transcriptomic pattems induced by the flb mutation in the fruit revealed the expression of a set of genes normally specific to the transition from flower to young berry development. Sorne of these genes, such as VvHBJ3, were functionally characterised, in particular through micro vine genetic transformation. Main outputs of this work will be presented and discussed.
DescripciónTrabajo presentado en el IX International Symposium on Grapevine Physiology and Biotechnology, celebrado en La Serena (Chile) del 21 al 26 de abril de 2013.-- Torregrosa, Laurent et al.
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