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In the microvine pericarp, critical transcriptome changes occur during the night

AutorRienth, Markus; Torregrosa, Laurent; Kelly, Mary T.; Luchaire, Nathalie; Grimplet, Jérôme ; Romieu, Charles
Fecha de publicaciónsep-2013
Citación2nd Annual ConferenceFleshy Fruit Development & Ripening (2013)
ResumenMany transcriptomic studies have been conducted on different vitis vinifera L. cultivars to better understand physiological changes occurring d uring berry development (Lijavetzky et al., 2012, Terrier et al., 2005, Pilati et al., 200 7). To our knowledge very few time course studies, assessing both day and night transcriptome , have been performed on any fleshy fruits until now. Still, it was demonstrated on mod el plants such as Arabidopsis thaliana (Schaffer et al., 2001, Chow and Kayb, 2013) that i mportant changes in gene expression following diurnal patterns occur in all living orga nisms and might influence organoleptic quality of the grapes. With the macrovine, which pr esents an annual reproductive cycle, studying day and night fruit development under cont rolled conditions is almost impossible. In our study, we useed the microvine (DRCF-mutant; Boss and Thomas, 2002) a recently proposed grapevine model (Chaib et al., 2010), whic h is a dwarf and present a rapid cycling and a continuous production of fruits. Gene express ion profile was studied on two pre - and two post-véraison stages of berry development using a whole genome microarray assay (Nimblegen® vitis 12x). Equivalent developmental st ages were sampled at day and night time. A total number of 9273 probesets were develop mentally modulated between all stages day and night with a high number of genes be ing modulated specifically at night (1755). Eight very similar clusters of gene express ion profiles could be obtained independently for day and night development. Our st udy allowed highlighting development stage specifics mechanisms of diurnal modulation of genes in the fruit that have been overlooked in other transcriptomic studies on fruit development so far. In the green berry functional enrichment analysis indicated major chan ges in cellular organization and photosynthesis whereas in the ripe berry mainly sec ondary metabolism was affected during the night.
DescripciónPóster presentado en la 2nd Annual ConferenceFleshy Fruit Development & Ripening (COST Action FA1106 QualityFruit), celebrada en Chania (Grecia) del 22 al 25 de septiembre de 2013.
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