Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/146840
COMPARTIR / EXPORTAR:
logo share SHARE logo core CORE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE

Invitar a revisión por pares abierta
Campo DC Valor Lengua/Idioma
dc.contributor.authorPuig-Castellví, Francesces_ES
dc.contributor.authorAlfonso, Ignacioes_ES
dc.contributor.authorPiña, Benjamínes_ES
dc.contributor.authorTauler, Romàes_ES
dc.date.accessioned2017-03-16T09:32:33Z-
dc.date.available2017-03-16T09:32:33Z-
dc.date.issued2016-08-03-
dc.identifier.citationScientific Reports 6: 30982 (2016)es_ES
dc.identifier.urihttp://hdl.handle.net/10261/146840-
dc.description.abstractDisruption of specific metabolic pathways constitutes the mode of action of many known toxicants and it is responsible for the adverse phenotypes associated to human genetic defects. Conversely, many industrial applications rely on metabolic alterations of diverse microorganisms, whereas many therapeutic drugs aim to selectively disrupt pathogens' metabolism. In this work we analyzed metabolic changes induced by auxotrophic starvation conditions in yeast in a non-targeted approach, using one-dimensional proton Nuclear Magnetic Resonance spectroscopy (1H NMR) and chemometric analyses. Analysis of the raw spectral datasets showed specific changes linked to the different stages during unrestricted yeast growth, as well as specific changes linked to each of the four tested starvation conditions (L-methionine, L-histidine, L-leucine and uracil). Analysis of changes in concentrations of more than 40 metabolites by Multivariate Curve Resolution-Alternating Least Squares (MCR-ALS) showed the normal progression of key metabolites during lag, exponential and stationary unrestricted growth phases, while reflecting the metabolic blockage induced by the starvation conditions. In this case, different metabolic intermediates accumulated over time, allowing identification of the different metabolic pathways specifically affected by each gene disruption. This synergy between NMR metabolomics and molecular biology may have clear implications for both genetic diagnostics and drug development. © The Author(s) 2016.es_ES
dc.description.sponsorshipThe research leading to these results has received funding from the European Research Council under the European Union's Seventh Framework Programme (FP/2007-2013) / ERC Grant Agreement n. 320737. We also thank Dr. Yolanda Pérez for her helpful recommendations on setting up the acquisition parameters for some of the NMR experiments.es_ES
dc.language.isoenges_ES
dc.publisherNature Publishing Groupes_ES
dc.relationinfo:eu-repo/grantAgreement/EC/FP7/320737es_ES
dc.relation.isversionofPostprintes_ES
dc.rightsopenAccesses_ES
dc.subjectMetabolic pathwayses_ES
dc.subjectNMR metabolomicses_ES
dc.title1H NMR metabolomic study of auxotrophic starvation in yeast using Multivariate Curve Resolution-Alternating Least Squares for Pathway Analysises_ES
dc.typeartículoes_ES
dc.identifier.doi10.1038/srep30982-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversion10.1038/srep30982es_ES
dc.contributor.funderEuropean Research Counciles_ES
dc.relation.csices_ES
oprm.item.hasRevisionno ko 0 false*
dc.identifier.funderhttp://dx.doi.org/10.13039/501100000781es_ES
dc.identifier.pmid27485935-
dc.type.coarhttp://purl.org/coar/resource_type/c_6501es_ES
item.openairetypeartículo-
item.cerifentitytypePublications-
item.languageiso639-1en-
item.grantfulltextopen-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextWith Fulltext-
Aparece en las colecciones: (IDAEA) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato
srep30982.pdf2,61 MBAdobe PDFVista previa
Visualizar/Abrir
Show simple item record

CORE Recommender

PubMed Central
Citations

5
checked on 23-mar-2024

SCOPUSTM   
Citations

28
checked on 22-mar-2024

WEB OF SCIENCETM
Citations

20
checked on 23-feb-2024

Page view(s)

299
checked on 28-mar-2024

Download(s)

235
checked on 28-mar-2024

Google ScholarTM

Check

Altmetric

Altmetric


Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.