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Development of multiplex serology platforms (bio-plex) for surveillance of diseases in wild boars in EU

AutorTouloudi, Antonia; Boadella, Mariana ; Gortázar, Christian
Fecha de publicación2012
Citación61st WDA/10th EWDA (2012)
ResumenThe potential of a multiplex serology test based on Luminex technology, where all antibodies are simultaneously detected in a single assay was investigated as a serodiagnostic tool for surveillance of diseases in wild boar population in Europe. The performance of «in-house> separate ELISAs which use relatively crude bacterial and cultured virus lysates as capture antigens was compared to the multiplex assay where the same antigens were covalently bound to the magnetic fluorescent beads used in the Luminex platform. Thirty six different bacterial, viral and parasitic antigens were tested with known hyper immune sera and with negative sera in serial dilutions. A panel of 483 wild boar sera of known positive and negative status for M. Bovis, Brucella spp, Hepatitis E virus and Aujeszky were used for validation purposes. The MPB83, BP 26, HEV 272 and Aujeszky gE components of the multiplex test respectively, showed similar sensitivities and specificities to the separate >in-house> ELISAs. In general, bacterial components performed well and the performance of the viral components was less successful which was attributed to relatively low signal strength for these antigens, leading to higher assay variability and a reduced ability to distinguish positive and negative samples compared to the >in-house> ELISAs. The use of recombinant proteins was investigated and was shown to lead to an appreciable high signal strength compared to the use of crude antigen in the Luminex system. The performance of the antigens in the Luminex assay was comparable to the WildTech serology array where the same antigens were spotted onto microarray strips.
DescripciónResumen del trabajo presentado a la 61st Wildlife Disease Association and 10th Biennial European Wildlife Disease Association: "convergence in wildlife health", celebrada en Lyon (Francia) del 23 al 27 de julio de 2012.-- et al.
URIhttp://hdl.handle.net/10261/146770
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