The effect of microsatellite selection on population genetic inference: insights from an empirical study using cross-specific and specific markers

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Campo DC	Valor	Lengua/Idioma
dc.contributor.author	Queirós, João	-
dc.contributor.author	Godinho, Raquel	-
dc.contributor.author	Gortázar, Christian	-
dc.contributor.author	Fuente, José de la	-
dc.contributor.author	Alves, Paulo C.	-
dc.date.accessioned	2017-03-13T11:57:26Z	-
dc.date.available	2017-03-13T11:57:26Z	-
dc.date.issued	2013	-
dc.identifier.citation	IMC11 (2013)	-
dc.identifier.uri	http://hdl.handle.net/10261/146640	-
dc.description	Resumen del trabajo presentado al 11th International Mammalogical Congress, celebrado en Belfast (Irlanda) del 11 al 16 de agosto de 2013.-- et al.	-
dc.description.abstract	Despite whole genome sequencing becoming accessible and feasible for non-model organisms, microsatellites remain the markers of choice to several population and conservation genetic studies. However, the selection criteria of microsatellites remain controversial due to the ascertainment bias that may be introduced into genetic inferences. We conducted an empirical study on red deer (Cervus elaphus) where we used cross-specific and specific microsatellites developed through pyrosequencing of enriched libraries. The comparison of these two datasets highlights the effect of specificity (cross-specific vs specific) and strategy (random vs non-random) of microsatellite selection on ecological and evolutionary inferences. We showed that good reliability and accuracy of genetic diversity could be achieved when using random markers, while reinforced previous findings of ascertainment bias in diversity when selecting the most polymorphic markers. Moreover, cross-specific amplifications biased genome-wide genetic diversity. Selecting appropriate microsatellite sets should primarily take into account the ecological and evolutionary questions.	-
dc.rights	closedAccess	-
dc.title	The effect of microsatellite selection on population genetic inference: insights from an empirical study using cross-specific and specific markers	-
dc.type	comunicación de congreso	-
dc.date.updated	2017-03-13T11:57:27Z	-
dc.description.version	Peer Reviewed	-
dc.language.rfc3066	eng	-
dc.relation.csic	Sí	-
dc.type.coar	http://purl.org/coar/resource_type/c_5794	es_ES
item.fulltext	No Fulltext	-
item.openairecristype	http://purl.org/coar/resource_type/c_18cf	-
item.cerifentitytype	Publications	-
item.grantfulltext	none	-
item.openairetype	comunicación de congreso	-
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