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The effect of microsatellite selection on population genetic inference: insights from an empirical study using cross-specific and specific markers

AuthorsQueirós, João ; Godinho, Raquel; Gortázar, Christian ; Fuente, José de la ; Alves, Paulo C.
Issue Date2013
CitationIMC11 (2013)
AbstractDespite whole genome sequencing becoming accessible and feasible for non-model organisms, microsatellites remain the markers of choice to several population and conservation genetic studies. However, the selection criteria of microsatellites remain controversial due to the ascertainment bias that may be introduced into genetic inferences. We conducted an empirical study on red deer (Cervus elaphus) where we used cross-specific and specific microsatellites developed through pyrosequencing of enriched libraries. The comparison of these two datasets highlights the effect of specificity (cross-specific vs specific) and strategy (random vs non-random) of microsatellite selection on ecological and evolutionary inferences. We showed that good reliability and accuracy of genetic diversity could be achieved when using random markers, while reinforced previous findings of ascertainment bias in diversity when selecting the most polymorphic markers. Moreover, cross-specific amplifications biased genome-wide genetic diversity. Selecting appropriate microsatellite sets should primarily take into account the ecological and evolutionary questions.
DescriptionResumen del trabajo presentado al 11th International Mammalogical Congress, celebrado en Belfast (Irlanda) del 11 al 16 de agosto de 2013.-- et al.
Appears in Collections:(IREC) Comunicaciones congresos
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