English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/146587
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Extending Immunological Profiling in the Gilthead Sea Bream, Sparus aurata, by Enriched cDNA Library Analysis, Microarray Design and Initial Studies upon the Inflammatory Response to PAMPs

AuthorsBoltaña, Sebastian; Novoa, Beatriz ; Figueras Huerta, Antonio ; MacKenzie, Simon
KeywordsExpressed sequence tags (EST)
Oligo-nucleotide microarray
Pathogen-associated molecular patterns (PAMPs)
Lipopolysaccharide (LPS)
Peptidoglycan (PGN)
Macrophages
Issue Date2017
PublisherMultidisciplinary Digital Publishing Institute
CitationInternational Journal of Molecular Sciences 18(2): 317 (2017)
AbstractThis study describes the development and validation of an enriched oligonucleotide-microarray platform for Sparus aurata (SAQ) to provide a platform for transcriptomic studies in this species. A transcriptome database was constructed by assembly of gilthead sea bream sequences derived from public repositories of mRNA together with reads from a large collection of expressed sequence tags (EST) from two extensive targeted cDNA libraries characterizing mRNA transcripts regulated by both bacterial and viral challenge. The developed microarray was further validated by analysing monocyte/macrophage activation profiles after challenge with two Gram-negative bacterial pathogen-associated molecular patterns (PAMPs; lipopolysaccharide (LPS) and peptidoglycan (PGN)). Of the approximately 10,000 EST sequenced, we obtained a total of 6837 EST longer than 100 nt, with 3778 and 3059 EST obtained from the bacterial-primed and from the viral-primed cDNA libraries, respectively. Functional classification of contigs from the bacterial- and viral-primed cDNA libraries by Gene Ontology (GO) showed that the top five represented categories were equally represented in the two libraries: metabolism (approximately 24% of the total number of contigs), carrier proteins/membrane transport (approximately 15%), effectors/modulators and cell communication (approximately 11%), nucleoside, nucleotide and nucleic acid metabolism (approximately 7.5%) and intracellular transducers/signal transduction (approximately 5%). Transcriptome analyses using this enriched oligonucleotide platform identified differential shifts in the response to PGN and LPS in macrophage-like cells, highlighting responsive gene-cassettes tightly related to PAMP host recognition. As observed in other fish species, PGN is a powerful activator of the inflammatory response in S. aurata macrophage-like cells. We have developed and validated an oligonucleotide microarray (SAQ) that provides a platform enriched for the study of gene expression in S. aurata with an emphasis upon immunity and the immune response
Description21 páginas, 7 figuras, 3 tablas.-- Sebastian Boltaña ... et al.-- This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license
Publisher version (URL)http://dx.doi.org/10.3390/ijms18020317
URIhttp://hdl.handle.net/10261/146587
DOI10.3390/ijms18020317
ISSN1661-6596
E-ISSN1422-0067
Appears in Collections:(IIM) Artículos
Files in This Item:
File Description SizeFormat 
Extending_immunological_profiling_2017.pdf4,84 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.