English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/146544
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

Biotechnological potential of Actinobacteria from Canadian and Azorean volcanic caves

AutorRiquelme, Cristina; Enes Dapkevicius, Maria de L. N.; Miller, A. Z. ; Charlop-Powers, Z.; Brady, Sean; Cohord; Cheeptham, N.
Palabras claveActinobacteria
Metagenomics
Antimicrobial activity
Enzymatic activity
Caves
Fecha de publicaciónene-2017
EditorSpringer
CitaciónApplied Microbiology and Biotechnology 101: 843- 857 (2017)
ResumenCaves are regarded as extreme habitats with appropriate conditions for the development of Actinobacteria. In comparison with other habitats, caves have not yet been the target of intensive screening for bioactive secondary metabolites produced by actinomycetes. As a primary screening strategy, we conducted a metagenomic analysis of the diversity and richness of a key gene required for non-ribosomal peptide (NRP) biosynthesis, focusing on cave-derived sediments from two Canadian caves (a lava tube and a limestone cave) to help us predict whether different types of caves may harbor drug-producing actinobacteria. Using degenerate PCR primers targeting adenylation domains (AD), a conserved domain in the core gene in NRP biosynthesis, a number of amplicons were obtained that mapped back to biomedically relevant NRP gene cluster families. This result guided our culture-dependent sampling strategy of actinomycete isolation from the volcanic caves of Canada (British Columbia) and Portugal (Azores) and subsequent characterization of their antibacterial and enzymatic activities. Multiple enzymatic and antimicrobial activities were identified from bacterial of the Arthrobacter and Streptomyces genera demonstrating that actinomycetes from volcanic caves are promising sources of antibacterial, antibiofilm compounds and industrially relevant enzymes. 15 páginas.-- 4 figuras.-- 3 tablas.-- referencias.-- The online version of this article (doi:10.1007/s00253-016-7932-7) contains supplementary material, which is available to authorized users.
Versión del editorhttp://dx.doi.org/10.1007/s00253-016-7932-7
URIhttp://hdl.handle.net/10261/146544
DOI10.1007/s00253-016-7932-7
Identificadoresdoi: 10.1007/s00253-016-7932-7
issn: 1432-0614
Aparece en las colecciones: (IRNAS) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
accesoRestringido.pdf15,38 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.