English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/146398
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Early transcriptional response to biotic stress in mixed starter fermentations involving Saccharomyces cerevisiae and Torulaspora delbrueckii

AuthorsTronchoni, Jordi ; Curiel, José Antonio ; Morales, Pilar ; Torres-Pérez, Rafael; González García, Ramón
KeywordsWine fermentation
Mixed starter
Non-Saccharomyces
Biotic stress
Interspecific interaction
Issue Date16-Jan-2017
PublisherElsevier
CitationInternational journal of food microbiology 241: 60-68 (2017)
AbstractAdvances in microbial wine biotechnology have led to the recent commercialization of several non-Saccharomyces starter cultures. These are intended to be used in either simultaneous or sequential inoculation with Saccharomyces cerevisiae. The different types of microbial interactions that can be stablished during wine fermentation acquire an increased relevance in the context of these mixed-starter fermentations. We analysed the transcriptional response to co-cultivation of S. cerevisiae and Torulaspora delbrueckii. The study focused in the initial stages of wine fermentation, before S. cerevisiae completely dominates the mixed cultures. Both species showed a clear response to the presence of each other, even though the portion of the genome showing altered transcription levels was relatively small. Changes in the transcription pattern suggested a stimulation of metabolic activity and growth, as a consequence of the presence of competitors in the same medium. The response of S. cerevisiae seems to take place earlier, as compared to T. delbrueckii. Enhanced glycolytic activity of the mixed culture was confirmed by the CO production profile during these early stages of fermentation. Interestingly, HSP12 expression appeared induced by co-cultivation for both of S. cerevisiae and Torulaspora delbrueckii in the two time points studied. This might be related with a recently described role of Hsp12 in intercellular communication in yeast. Expression of S. cerevisiae PAU genes was also stimulated in mixed cultures.
Publisher version (URL)http://doi.org/10.1016/j.ijfoodmicro.2016.10.017
URIhttp://hdl.handle.net/10261/146398
DOI10.1016/j.ijfoodmicro.2016.10.017
Identifiersissn: 0168-1605
Appears in Collections:(ICVV) Artículos
Files in This Item:
File Description SizeFormat 
Tronchoni et al III.docx77,31 kBMicrosoft Word XMLView/Open
Figure1 IJFM.pdf214,92 kBAdobe PDFThumbnail
View/Open
Figure2 IJFM.pdf201,54 kBAdobe PDFThumbnail
View/Open
Figure3 IJFM.pdf314,38 kBAdobe PDFThumbnail
View/Open
Figure4 IJFM.pdf176,75 kBAdobe PDFThumbnail
View/Open
Figure5 IJFM.pdf73,87 kBAdobe PDFThumbnail
View/Open
Supplementary File S1.pdf143,44 kBAdobe PDFThumbnail
View/Open
Supplementary File S2.xlsx7,5 MBMicrosoft Excel XMLView/Open
Supplementary File S3.pdf217,02 kBAdobe PDFThumbnail
View/Open
Supplementary File S4.pdf177,87 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.