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Electronic volume increases during capacitation in ram spermatozoa

AutorOlmo, Enrique del ; García-Álvarez, Olga; Maroto-Morales, Alejandro ; Ramón, Manuel; Jiménez-Rabadán, Pilar; Anel-López, Luis; Soler, Ana J. ; Garde, José Julián ; Fernández-Santos, M. Rocío
Fecha de publicación2014
CitaciónAERA 2014
ResumenCapacitation is a complex process that involves multiple changes in sperm physiology. One aspect of these changes is an increasing destabilization which prepares the membrane for the acrosome reaction and fusion with the oocyte. An important likely consequence of the changes in sperm plasma membrane function is a change in ion transport regulation, resulting in a change in intracellular ionic concentrations. We can monitor changes in sperm volume by flow cytometry. We have evaluated the effect of capacitation on volume of ram spermatozoa by means of CellLab QUANTA SC cytometer which can estimate cell volume due to the electrical resistance that produces spermatozoa as they passes through a capillary (Electronic Volume). Thawed sperm samples form 16 rams were incubated at 38.5°C and 5% CO2 in SOF-HEPES (287 mOsm) or SOF-HEPES with 10% of oestrum sheep serum (OSS; 295 mOsm). Sperm volume was analyzed before and after 30 min of incubation. Changes in the Electronic Volume were assessed in viable spermatozoa with intact acrosome (PI-/PNA-) and were shown as the rate of volume increase (DEV). Spermatozoa incubated with OSS showed a higher DEV than no-OSS incubation treatment (7.53 +/- 1.12% and 1.75 +/- 1.12%, respectively, p < 0.001). Our results show that capacitation causes an increase of sperm electronic volume of ram spermatozoa. Anyway, further studies should be carried out to determine the processes involved in this increase of volume.
DescripciónResumen del póster presentado al 12th International Congress of the Spanish Association of Animal Reproduction, celebrado en Alicante del 16 al 18 de octubre de 2014.
URIhttp://hdl.handle.net/10261/146395
Aparece en las colecciones: (IREC) Comunicaciones congresos
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