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Anaplasma phagocytophilum strains inhibit apoptosis of Ixodes spp. tick cells to enhance early survival and multiplication

AutorAlberdi, Pilar ; Ayllón, Nieves ; Martínez de Carnero, Mª Luisa; Bell-Sakyi, Lesley; Fuente, José de la
Fecha de publicación2014
CitaciónTTP8 & STVM12 (2014)
Resumen[Objectives]: Anaplasma phagocytophilum (Ap) is an intracellular rickettsial pathogen transmitted by Ixodes spp. ticks, which causes granulocytic anaplasmosis in humans, horses and dogs and tick-borne fever (TBF) in ruminants. In the United States, human granulocytic anaplasmosis (HGA) is highly prevalent while TBF has not been reported. However, in Europe the situation is the opposite, with high prevalence of TBF in sheep and low prevalence of HGA. The origin of these differences has not been identified and is the focus of this research. [Method]: In the invertebrate host, Ap multiplies within a parasitophorous vacuole, thus evading host defenses. In this study we used three different strains of Ap of human, canine and ovine origin to infect the Ixodes ricinus-derived cell line IRE/CTVM20 and the Ixodes scapularis-derived cell line ISE6 to observe the effect of infection on the level of apoptosis in the cells. Inhibition of apoptosis was observed by flow cytometry as early as 24h after infection of both tick cell lines with all three strains of Ap, suggesting that the infection with Ap inhibits the intrinsic apoptosis pathway independently of the origin of Ap strains and vector species. [Conclusions]: These results increase our understanding of the mechanisms of Ap infection and multiplication and suggest that other mechanisms affect disease prevalence in different regions.
DescripciónResumen del póster presentado al Joint 8th International Ticks and Tick-borne Pathogens and 12th Biennial Society for Tropical Veterinary Medicine Conference, celebradas en Cape Town (Sudafrica) del 24 al 29 de agosto de 2014.
URIhttp://hdl.handle.net/10261/146355
Aparece en las colecciones: (IREC) Comunicaciones congresos
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