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Title

The influence of the carrier molecule on amoxicillin recognition by specific IgE in patients with immediate hypersensitivity reactions to betalactams

AuthorsAriza, Adriana; Mayorga, Cristobalina; Salas, María; Doña, Inmaculada; Martín-Serrano, Ángela; Pérez-Inestrosa, Ezequiel; Pérez-Sala, Dolores CSIC ORCID ; Guzmán, Antonio E.; Montañez, M. I.; Torres, María J.
Issue Date12-Oct-2016
PublisherNature Publishing Group
CitationScientific Reports 6:35113 (2016)
AbstractThe optimal recognition of penicillin determinants, including amoxicillin (AX), by specific IgE antibodies is widely believed to require covalent binding to a carrier molecule. The nature of the carrier and its contribution to the antigenic determinant is not well known. Here we aimed to evaluate the specific-IgE recognition of different AX-derived structures. We studied patients with immediate hypersensitivity reactions to AX, classified as selective or cross-reactors to penicillins. Competitive immunoassays were performed using AX itself, amoxicilloic acid, AX bound to butylamine (AXO-BA) or to human serum albumin (AXO-HSA) in the fluid phase, as inhibitors, and amoxicilloyl-poli-L-lysine (AXO-PLL) in the solid-phase. Two distinct patterns of AX recognition by IgE were found: Group A showed a higher recognition of AX itself and AX-modified components of low molecular weights, whilst Group B showed similar recognition of both unconjugated and conjugated AX. Amoxicilloic acid was poorly recognized in both groups, which reinforces the need for AX conjugation to a carrier for optimal recognition. Remarkably, IgE recognition in Group A (selective responders to AX) is influenced by the mode of binding and/or the nature of the carrier; whereas IgE in Group B (cross-responders to penicillins) recognizes AX independently of the nature of the carrier.
Description10 p.-4 fig.-1 tab.
Publisher version (URL)http://dx.doi.org/10.1038/srep35113
URIhttp://hdl.handle.net/10261/146339
DOI10.1038/srep35113
ISSN2045-2322
E-ISSN2045-2322
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