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Title

From dirt to industrial applications: Pseudomonas putida as a Synthetic Biology chassis for hosting harsh biochemical reactions

AuthorsNikel, Pablo I.; Chavarría, Max; Danchin, Antoine; Fraile, Sofía; Lorenzo, Víctor de
KeywordsPseudomonas putida
Issue Date27-May-2016
PublisherElsevier
CitationCurrent Opinion in Chemical Biology 34: 20–29 (2016)
AbstractThe soil bacterium Pseudomonas putida is endowed with a central carbon metabolic network capable of fulfilling high demands of reducing power. This situation arises from a unique metabolic architecture that encompasses the partial recycling of triose phosphates to hexose phosphates-the so-called EDEMP cycle. In this article, the value of P. putida as a bacterial chassis of choice for contemporary, industrially-oriented metabolic engineering is addressed. The biochemical properties that make this bacterium adequate for hosting biotransformations involving redox reactions as well as toxic compounds and intermediates are discussed. Finally, novel developments and open questions in the continuous quest for an optimal microbial cell factory are presented at the light of current and future needs in the area of biocatalysis.
Publisher version (URL)http://dx.doi.org/10.1016/j.cbpa.2016.05.011
URIhttp://hdl.handle.net/10261/146304
DOI10.1016/j.cbpa.2016.05.011
ISSN1367-5931
E-ISSN1879-0402
Appears in Collections:(CNB) Artículos
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