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Título

Study of adsorption and penetration of E2(279-298) peptide into Langmuir phospholipid monolayers

AutorLarios, Cristina; Miñones Jr., J.; Haro Villar, Isabel; Alsina, M. Asunción; Busquets, M. Antònia; Miñones Trillo, J.
Palabras claveHepatitis G
Infección vírica
Péptidos
Fecha de publicación25-oct-2006
EditorAmerican Chemical Society
CitaciónThe Journal of Physical Chemistry B 110(46): 23292-23299 (2006)
ResumenThe peptide corresponding to the sequence (279-298) of the Hepatitis G virus (HGV/GBV-C) E2 protein was synthesized, and surface activity measurements, π-A compression isotherms, and penetration of E2- (279-298) into phospholipid monolayers spread at the air-water interface were carried out on water and phosphate buffer subphases. The results obtained indicated that the pure E2(279-298) Langmuir monolayer exhibited a looser packing on saline-buffered than on pure water subphase and suggest that the increase in subphase ionic strength stabilizes the peptide monolayer. To better understand the topography of the monolayer, Brewster angle microscopy (BAM) images of pure peptide monolayers were obtained. Penetration of the peptide into the pure lipid monolayers of dipalmitoylphosphatidylcholine (DPPC) and dimyristoylphosphatidylcholine (DMPC) and into mixtures of dimyristoylphosphatidylcholine/dimyristoylphosphatidylglycerol (DMPC/DMPG) at various initial surface pressures was investigated to determine the ability of these lipid monolayers to host the peptide. The higher penetration of peptide into phospholipids is attained when the monolayers are in the liquid expanded state, and the greater interaction is observed with DMPC. Furthermore, the penetration of the peptide dissolved in the subphase into these various lipid monolayers was investigated to understand the interactions between the peptide and the lipid at the air-water interface. The results obtained showed that the lipid acyl chain length is an important parameter to be taken into consideration in the study of peptide-lipid interactions.
Descripción8 pages, 10 figures.-- PMID: 17107178 [PubMed].-- Printed version published Nov 23, 2006.-- Supporting information available at: http://pubs.acs.org/doi/full/10.1021/jp0628582
Versión del editorhttp://dx.doi.org/10.1021/jp0628582
URIhttp://hdl.handle.net/10261/14546
DOI10.1021/jp0628582
ISSN1520-5207
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