English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/145424
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Evolutionary functional elaboration of the Elovl2/5 gene family in chordates

AuthorsMonroig, Óscar ; Lopes-Marques, Mónica; Navarro, Juan Carlos ; Hontoria, Francisco ; Ruivo, Raquel; Santos, Miguel M.; Venkatesh, Byrappa; Tocher, Douglas R.; Castro, L. Filipe C.
Issue Date9-Feb-2016
PublisherNature Publishing Group
CitationScientific Reports 6: 20510 (2016)
AbstractThe biosynthesis of long-chain polyunsaturated fatty acids (LC-PUFA) provides an intriguing example on how multi-enzymatic cascades evolve. Essential LC-PUFA, such as arachidonic, eicosapentaenoic, and docosahexaenoic acids (DHA), can be acquired from the diet but are also endogenously retailored from C precursors through consecutive elongations and desaturations catalyzed, respectively, by fatty acyl elongase and desaturase enzymes. The molecular wiring of this enzymatic pathway defines the ability of a species to biosynthesize LC-PUFA. Exactly when and how in animal evolution a functional LC-PUFA pathway emerged is still elusive. Here we examine key components of the LC-PUFA cascade, the Elovl2/Elovl5 elongases, from amphioxus, an invertebrate chordate, the sea lamprey, a representative of agnathans, and the elephant shark, a basal jawed vertebrate. We show that Elovl2 and Elovl5 emerged from genome duplications in vertebrate ancestry. The single Elovl2/5 from amphioxus efficiently elongates C and C and, to a marked lesser extent, C LC-PUFA. Lamprey is incapable of elongating C substrates. The elephant shark Elovl2 showed that the ability to efficiently elongate C PUFA and thus to synthesize DHA through the Sprecher pathway, emerged in the jawed vertebrate ancestor. Our findings illustrate how non-integrated >metabolic islands> evolve into fully wired pathways upon duplication and neofunctionalization.
Publisher version (URL)https://doi.org/10.1038/srep20510
URIhttp://hdl.handle.net/10261/145424
DOI10.1038/srep20510
Identifiersissn: 2045-2322
Appears in Collections:(IATS) Artículos
Files in This Item:
File Description SizeFormat 
Evolutionary functional elaboration-Monroig.pdf1,48 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.