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Title: | Solutions to decrease a systematic error related to AAPH addition in the fluorescence-based ORAC assay |
Authors: | Mellado-Ortega, Elena; Zabalgogeazcoa, Iñigo ; Vázquez de Aldana, Beatriz R. ![]() ![]() |
Keywords: | AAPH ORAC Peroxyl radical Total antioxidant capacity Trolox 96-well plate reader |
Issue Date: | 2016 |
Publisher: | Elsevier |
Citation: | Analytical Biochemistry (519): 27-29 (2017) |
Abstract: | Oxygen radical absorbance capacity (ORAC) assay in 96-well multi-detection plate readers is a rapid method to determine total antioxidant capacity (TAC) in biological samples. A disadvantage of this method is that the antioxidant inhibition reaction does not start in all of the 96 wells at the same time due to technical limitations when dispensing the free radical-generating azo initiator 2,2′-azobis(2-methyl propanimidamide) dihydrochloride (AAPH). The time delay between wells yields a systematic error that causes statistically significant differences in TAC determination of antioxidant solutions depending on their plate position. We propose two alternative solutions to avoid this AAPH-dependent error in ORAC assays. |
Description: | 8 páginas, 1 figura, 1 tabla. -- The definitive version is available at https://www.elsevier.com |
Publisher version (URL): | http://dx.doi.org/10.1016/j.ab.2016.12.009 |
URI: | http://hdl.handle.net/10261/144182 |
DOI: | http://dx.doi.org/10.1016/j.ab.2016.12.009 |
ISSN: | 0003-2697 |
E-ISSN: | 1096-0309 |
Appears in Collections: | (IRNASA) Artículos |
Files in This Item:
File | Description | Size | Format | |
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Solutions to decrease a systematic error related to AAPH addition in the fluorescence-based ORAC assay.docx | Artículo principal | 135,3 kB | Microsoft Word XML | View/Open |
Supplementary material_Solutions to decrease a systematic error related to AAPH addition in the fluorescence-based ORAC assay.docx | Material suplementario | 69,93 kB | Microsoft Word XML | View/Open |