English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/144182
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:

Title

Solutions to decrease a systematic error related to AAPH addition in the fluorescence-based ORAC assay

AuthorsMellado-Ortega, Elena; Zabalgogeazcoa, Iñigo ; Vázquez de Aldana, Beatriz R. ; Arellano, Juan B.
KeywordsAAPH
ORAC
Peroxyl radical
Total antioxidant capacity
Trolox
96-well plate reader
Issue Date2016
PublisherElsevier
CitationAnalytical Biochemistry (519): 27-29 (2017)
AbstractOxygen radical absorbance capacity (ORAC) assay in 96-well multi-detection plate readers is a rapid method to determine total antioxidant capacity (TAC) in biological samples. A disadvantage of this method is that the antioxidant inhibition reaction does not start in all of the 96 wells at the same time due to technical limitations when dispensing the free radical-generating azo initiator 2,2′-azobis(2-methyl propanimidamide) dihydrochloride (AAPH). The time delay between wells yields a systematic error that causes statistically significant differences in TAC determination of antioxidant solutions depending on their plate position. We propose two alternative solutions to avoid this AAPH-dependent error in ORAC assays.
Description8 páginas, 1 figura, 1 tabla. -- The definitive version is available at https://www.elsevier.com
Publisher version (URL)http://dx.doi.org/10.1016/j.ab.2016.12.009
URIhttp://hdl.handle.net/10261/144182
DOIhttp://dx.doi.org/10.1016/j.ab.2016.12.009
ISSN0003-2697
E-ISSN1096-0309
Appears in Collections:(IRNASA) Artículos