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Characterization of pseudorabies virus of wild boar origin from Europe

AuthorsMüller, T.; Ruiz Fons, Francisco ; Gortázar, Christian
Aujeszky’s disease
Wild boar
Pseudorabies virus
Molecular epidemiology
Restriction fragment length polymorphism
Issue Date2010
PublisherCambridge University Press
CitationEpidemiology and Infection 138(11): 1590-1600 (2010)
AbstractPseudorabies virus (PrV) infections appear to be more widely distributed in the European wild boar (Sus scrofa) population than assumed. In Europe, attempts to isolate and characterize the causative agents have been limited so far. We therefore collected and examined a total of 35 PrV isolates obtained from wild boar or hunting dogs in Germany, France, Spain, Italy, Slovakia and Hungary between 1993 and 2008. Restriction enzyme analysis of genomic DNA using BamHI showed that all isolates, except one, belonged to genogroup I but different subtypes were evident. For further investigations of the phylogenetic relationships, a 732-bp fragment of the glycoprotein C (gC) gene was amplified by PCR. Sequence analysis revealed about 40 variant positions within this fragment. Comparison of the nucleotide sequences supported the separation into a clade containing isolates from North-Rhine Westphalia, Rhineland-Palatinate (Germany), France and Spain (clade B) and an apparently more variable clade comprising isolates from Brandenburg, Baden-Wurttemberg, Saxony, Saxony-Anhalt (Germany), Slovakia, Hungary, Italy and France (clade A).
Identifiersdoi: 10.1017/S0950268810000361
issn: 0950-2688
e-issn: 1469-4409
Appears in Collections:(IREC) Artículos
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