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Title

Study on extraction, purification and characterization of a novel peroxidase from white Spanish broom (Cytisus multiflorus)

AuthorsPérez Galende, Patricia; García de María, Cándido; Arellano, Juan B. ; Roig, Manuel G.; Shnyrov, Valery L.
Keywordsperoxidase
protein purification
Cytisus multiflorus
chromatography
Issue DateSep-2016
PublisherJSciMed Central
AbstractPeroxidases (EC 1.11.1.7) are a large group of enzymes widely distributed in the plant kingdom. The present work describes a study on the isolation, purification and some features of a novel peroxidase from white Spanish broom (Cytisus multiflorus), a tree legume very abundant in the northern half of Spain and Portugal. Optimal conditions are proposed for enzyme extraction, removal of phenolic compounds and enzyme purification by consecutive hydrophobic, ion-exchange and size-exclusion chromatographies. Peroxidase from Cytisus multiflorus (CMP) was found to have a molecular weight of 49 kDa. The spectrum of CMP showed a Soret band at 403 nm with a Rz factor of 3.3. Substrate specificity and the effect of some variables on the activity of CMP with guaiacol as cosubstrate have also been investigated.
Publisher version (URL)https://www.jscimedcentral.com/PlantBiology/plantbiology-4-1052.pdf
URIhttp://hdl.handle.net/10261/144106
ISSN2333-6668
Appears in Collections:(IRNASA) Artículos
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