Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/144066
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Muriach, Borja | - |
dc.contributor.author | Carrillo, Manuel | - |
dc.contributor.author | Zanuy, Silvia | - |
dc.contributor.author | Cerdá-Reverter, José Miguel | - |
dc.date.accessioned | 2017-02-16T09:52:35Z | - |
dc.date.available | 2017-02-16T09:52:35Z | - |
dc.date.issued | 2014-06 | - |
dc.identifier | issn: 0920-1742 | - |
dc.identifier.citation | Fish Physiology and Biochemistry 40: 849-864 (2014) | - |
dc.identifier.uri | http://hdl.handle.net/10261/144066 | - |
dc.description.abstract | The sea bass follicle-stimulating hormone 5′ flanking region (sbFSHβ 5′ FR) was cloned and characterized in order to study the molecular mechanisms underlying transcriptional regulation of the sbFSHβ gene. Analysis of the ~3.5 kb of this region revealed the presence of several putative cis-acting elements, including steroid hormone response elements, cAMP response elements, pituitary-specific transcription factor response elements, activator protein-1 response elements and TATA sequence. Deleted constructs containing ~3.5 kb of the sbFSHβ 5′ FR fused to a luciferase reporter gene were transiently transfected into human embryonic kidney (HEK 293) and mouse mature gonadotrope (LβT2) cell lines. The sbFSHβ 5′ FR was efficiently expressed under basal conditions in LβT2 but not in HEK 293, pointing to both positive and negative regulatory elements. In order to elucidate the estrogen-mediated sbFSHβ transcriptional activity, in vitro treatments with 17β-estradiol were carried out on primary cultures of pituitary cells and LβT2 cells transiently expressing luciferase under the control of sbFSHβ 5′ FR. Overall, these results demonstrate that 17β-estradiol inhibits sbFSHβ gene expression directly at the level of the pituitary. However, it was also shown that estrogen did not induce changes of the sbFSH promoter-directed luciferase activity, suggesting that sbFSHβ 5′FR (~3.5 kb) activity is cell type dependent and its estrogen regulation could require cis-acting elements located upstream of the promoter region, which is characterized in this article. © 2013 Springer Science+Business Media Dordrecht. | - |
dc.description.sponsorship | This work was supported by EU (QLRT-2001-01801) and MEC (AGL-2005-00796) grants. During this work, Borja Muriach was the recipient of an I3P Predoctoral Fellowship from Consejo Superior de Investigaciones Científicas (CSIC). During part of this research, JMC-R was financed by AGL2010-22247-C03-01 grant and JMC-R and SZ by CSD 2007-00002 grant. | - |
dc.publisher | Springer Nature | - |
dc.relation.isversionof | Postprint | - |
dc.rights | closedAccess | - |
dc.subject | FSHβ | - |
dc.subject | Promoter analysis | - |
dc.subject | Fish | - |
dc.subject | Estrogen receptor | - |
dc.subject | LβT2 | - |
dc.subject | Reproduction | - |
dc.title | Characterization of sea bass FSHβ 5′ flanking region: Transcriptional control by 17β-estradiol | - |
dc.type | artículo | - |
dc.identifier.doi | 10.1007/s10695-013-9891-6 | - |
dc.relation.publisherversion | https://doi.org/10.1007/s10695-013-9891-6 | - |
dc.date.updated | 2017-02-16T09:52:35Z | - |
dc.description.version | Peer Reviewed | - |
dc.language.rfc3066 | eng | - |
dc.contributor.funder | Ministerio de Economía y Competitividad (España) | - |
dc.contributor.funder | Consejo Superior de Investigaciones Científicas (España) | - |
dc.contributor.funder | European Commission | - |
dc.contributor.funder | Ministerio de Educación y Ciencia (España) | - |
dc.relation.csic | Sí | - |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003329 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100003339 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/501100000780 | es_ES |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | No Fulltext | - |
Aparece en las colecciones: | (IATS) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
accesoRestringido.pdf | 15,38 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
4
checked on 12-abr-2024
WEB OF SCIENCETM
Citations
4
checked on 27-feb-2024
Page view(s)
297
checked on 18-abr-2024
Download(s)
96
checked on 18-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.