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dc.contributor.authorBardaji, Leirees_ES
dc.contributor.authorAñorga, Maitees_ES
dc.contributor.authorRuiz-Masó, José A.es_ES
dc.contributor.authorSolar, Gloria deles_ES
dc.contributor.authorMurillo, Jesúses_ES
dc.date.accessioned2017-02-15T11:56:10Z-
dc.date.available2017-02-15T11:56:10Z-
dc.date.issued2017-02-13-
dc.identifier.citationFrontiers in Microbiology 8:190 (2017)es_ES
dc.identifier.urihttp://hdl.handle.net/10261/144008-
dc.description14 p.-5 figes_ES
dc.description.abstractPlasmids are a main factor for the evolution of bacteria through horizontal gene exchange, including the dissemination of pathogenicity genes, resistance to antibiotics and degradation of pollutants. Their capacity to duplicate is dependent on their replication determinants (replicon), which also define their bacterial host range and the inability to coexist with related replicons. We characterize a second replicon from the virulence plasmid pPsv48C, from Pseudomonas syringae pv. savastanoi, which appears to be a natural chimera between the gene encoding a newly described replication protein and a putative replication control region present in the widespread family of PFP virulence plasmids. We present extensive evidence of this type of chimerism in structurally similar replicons from species of Pseudomonas, including environmental bacteria as well as plant, animal and human pathogens. We establish that these replicons consist of two functional modules corresponding to putative control (REx-C module) and replication (REx-R module) regions. These modules are functionally separable, do not show specificity for each other, and are dynamically exchanged among replicons of four distinct plasmid families. Only the REx-C module displays strong incompatibility, which is overcome by a few nucleotide changes clustered in a stem-and-loop structure of a putative antisense RNA. Additionally, a REx-C module from pPsv48C conferred replication ability to a non-replicative chromosomal DNA region containing features associated to replicons. Thus, the organization of plasmid replicons as independent and exchangeable functional modules is likely facilitating rapid replicon evolution, fostering their diversification and survival, besides allowing the potential co-option of appropriate genes into novel replicons and the artificial construction of new replicon specificities.es_ES
dc.description.sponsorshipThis work was funded by the Spanish Plan Nacional I+D+i grant AGL2014-53242-C2-2-R, fromthe Ministerio de Economía y Competitividad (MINECO), co-financed by the Fondo Europeo de Desarrollo Regional (FEDER). M.A. was supported by an FPI fellowship (reference BES-2012-054016, Ministerio de Ciencia e Innovación/Ministerio de Economía y Competitividad, Spain).es_ES
dc.language.isoenges_ES
dc.publisherFrontiers Mediaes_ES
dc.relation.isversionofPublisher's versiones_ES
dc.rightsopenAccesses_ES
dc.subjectControl and replication moduleses_ES
dc.subjectChimeric repliconses_ES
dc.subjectGene co-optiones_ES
dc.subjectRep proteinses_ES
dc.subjectOrigin of replicationes_ES
dc.subjectPlasmid incompatibilityes_ES
dc.subjectSwapping of functional moduleses_ES
dc.subjectVirulence plasmidses_ES
dc.titlePlasmid replicons from Pseudomonas are natural chimeras of functional, exchangeable moduleses_ES
dc.typeartículoes_ES
dc.identifier.doi10.3389/fmicb.2017.00190-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://dx.doi.org/10.3389/fmicb.2017.00190es_ES
dc.identifier.e-issn1664-302X-
dc.rights.licensehttps://creativecommons.org/licenses/by/4.0/es_ES
dc.contributor.funderMinisterio de Economía y Competitividad (España)es_ES
dc.relation.csices_ES
oprm.item.hasRevisionno ko 0 false*
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003329es_ES
dc.identifier.pmid28243228-
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