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dc.contributor.authorAgulló, Loreinees_ES
dc.contributor.authorRomero-Silva, María Josées_ES
dc.contributor.authorDomenech, Mirianes_ES
dc.contributor.authorSeeger, Michaeles_ES
dc.date.accessioned2017-02-15T09:31:04Z-
dc.date.available2017-02-15T09:31:04Z-
dc.date.issued2017-01-10-
dc.identifier.citationPLoS ONE 12(1): e0169544 (2017)es_ES
dc.identifier.urihttp://hdl.handle.net/10261/143971-
dc.description24 p.-8 fig.-3 tab.es_ES
dc.description.abstractp-Cymene is an aromatic terpene that is present in diverse plant species. The aims of this study were to study the p-cymene metabolism in the model aromatic-degrading bacterium Burkholderia xenovorans LB400, and its response to p-cymene. The catabolic p-cymene (cym) and p-cumate (cmt) genes are clustered on the LB400 major chromosome. B. xenovorans LB400 was able to grow on p-cymene as well as on p-cumate as a sole carbon and energy sources. LB400 growth attained higher cell concentration at stationary phase on p-cumate than on p-cymene. The transcription of the key cymAb and cmtAb genes, and p-cumate dioxygenase activity were observed in LB400 cells grown on p-cymene and on p-cumate, but not in glucose-grown cells. Diverse changes on LB400 proteome were observed in p-cymene-grown cells compared to glucose-grown cells. An increase of the molecular chaperones DnaK, GroEL and ClpB, the organic hydroperoxide resistance protein Ohr, the alkyl hydroperoxide reductase AhpC and the copper oxidase CopA during growth on p-cymene strongly suggests that the exposure to p-cymene constitutes a stress condition for strain LB400. Diverse proteins of the energy metabolism such as enolase, pyruvate kinase, aconitase AcnA, succinyl-CoA synthetase beta subunit and ATP synthase beta subunit were induced by p-cymene. Electron microscopy showed that p-cymene-grown cells exhibited fuzzy outer and inner membranes and an increased periplasm. p-Cymene induced diverse membrane and transport proteins including the p-cymene transporter CymD. Biofilm formation was reduced during growth in p-cymene in strain LB400 compared to glucose-grown cells that may be associated with a decrease of diguanylate cyclase protein levels. Overall, these results indicate active p-cymene and p-cumate catabolic pathways in B. xenovorans LB400. In addition, this study showed that p-cymene activated a stress response in strain LB400 and reduced its biofilm formation.es_ES
dc.description.sponsorshipFONDECYT 1110992 and 1151174 to Michael Seeger. USM 131342 and 131562 to Michael Seeger. MS acknowledges support from FONDECYT (1110992 and 1151174) (http://www.fondecyt.cl) and USM (131342 and 131562) (http://www.usm.cl) grants.es_ES
dc.language.isoenges_ES
dc.publisherPublic Library of Sciencees_ES
dc.relation.isversionofPublisher's versiones_ES
dc.rightsopenAccesses_ES
dc.titlep-Cymene promotes Its catabolism through the p-Cymene and the p-Cumate pathways, activates a stress response and reduces the biofilm formation in Burkholderia xenovorans LB400es_ES
dc.typeartículoes_ES
dc.identifier.doi10.1371/journal.pone.0169544-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1371/journal.pone.0169544es_ES
dc.identifier.e-issn1932-6203-
dc.rights.licensehttps://creativecommons.org/licenses/by/4.0/es_ES
dc.contributor.funderFondo Nacional de Desarrollo Científico y Tecnológico (Chile)es_ES
dc.relation.csices_ES
oprm.item.hasRevisionno ko 0 false*
dc.identifier.funderhttp://dx.doi.org/10.13039/501100002850es_ES
dc.identifier.pmid28072820-
dc.type.coarhttp://purl.org/coar/resource_type/c_6501es_ES
item.openairetypeartículo-
item.cerifentitytypePublications-
item.languageiso639-1en-
item.grantfulltextopen-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextWith Fulltext-
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