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Effect of nutritional interventions on immune response and gut health of gilthead sea bream (sparus aurata)

AutorSitjà-Bobadilla, Ariadna ; Estensoro, Itziar ; Calduch-Giner, Josep A. ; Pérez-Sánchez, Jaume
Fecha de publicaciónsep-2015
Citación9th International Symposium on Fish Parasites (2015)
ResumenAquaculture sustainability of most Mediterranean fish species relies on plant ingredients as an alternative to fish protein and fish oil (FO) for aquafeeds, but the detrimental health effects can limit replacement levels. Feed additives have been proposed as an alternative to mitigate negative effects of anti-nutritional factors and extend opportunities for replacement of ingredients from finite marine resources. We compile here the results of a series of studies with different feed additives in juvenile gilthead sea bream (GSB). The additives tested were: 1) a sodium butyrate preparation (BP- 70®Norel) and 2) a combination of the essential oils carvacrol and thymol, NEXT ENHANCE®150 (Novus, NE), with or without a prebiotic (Previda®, PRE). The effects of BP-70 added at 0.4% to a diet in which FO was replaced at 84% by a blend of vegetable oils (VOs), were compared with a control diet with no FO replacement, and another diet replacing VOs at 58%. NE was given at 100 ppm alone or in combination with PRE (0.5%) and compared to a control diet. For both products, gut health was evaluated histologically, immunocytochemically and at the transcriptomic level with a 96-well PCR array for 86 genes, including markers of: cell differentiation and proliferation, intestinal architecture and permeability, enterocyte mass/function and epithelial damage, immunesurveillance (interleukins, cytokines and chemokines receptors, pattern recognition receptors), and mitochondria function and biogenesis. For BP-70 (added at 0.8%), the effects on innate humoral parameters, intestinal microbiota and survival after a challenge with Photobacterium damselae subsp. piscicida were also evaluated. The expression profile in fish fed the control and the low substitution diet was almost equal, whereas a wide range of markers including those of cell proliferation and differentiation, intestinal barrier function antioxidant defence, interleukin function and lectin recognition system were overexpressed in the extreme diet fish. However, this expression pattern was almost reversed to the gene expression profile of control fish with butyrate supplementation. Diets with NE and/or PRE provoked significant changes in the expression of 26 genes, inducing an anti-inflammatory and anti-proliferative transcriptomic profile. An apparent benefit of BP-70 inclusion in terms of stimulation of the innate immune response and protection against photobacteriosis was also observed. The lower microbial diversity observed in BP-70 fed fish could indicate that their intestinal microbiota achieves a stable situation faster than that from control animals. Butyrate supplementation is a promising strategy for GSB, especially when fish are facing stress associated with sustainable diets with extremely low inclusion levels of fish meal and FO. The dietary combination of NE and PRE could have a potential use for overcoming some types of nutritionally or pathologically induced gut inflammation.
DescripciónComunicación presentada en el 9th International Symposium on Fish Parasites, celebrado en Valencia, España, del 31 de agosto al 4 de septiembre de 2015
URIhttp://hdl.handle.net/10261/143921
Aparece en las colecciones: (IATS) Comunicaciones congresos
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