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Differential humoral immune response of gilthead sea bream (sparus aurata) resistant to enteromyxum leei (myxozoa) re-infection

AuthorsEstensoro, Itziar ; Pozo, R. del; Sitjà-Bobadilla, Ariadna
Issue Date7-Sep-2015
Citation9th International Symposium on Fish Parasites (2015)
AbstractEnteromyxosis in gilthead sea bream (GSB) provokes severe chronic enteritis leading to emaciation, caquexia and high mortalities. This study focuses on the humoral immune response of GSB, by the detection of the specific circulating antibodies and the measurement of serum peroxidases. Survivor GSB from previous E. leei experimental infections (n = 21) and naïve GSB (n = 20) were equally distributed into two tanks and exposed to E. leei-contaminated water effluent from a donor tank during 175 days. Blood samples were taken at 0, 66, 105 and 175 days post exposure (dpe) from both groups and sera obtained. Parasite diagnosis was performed by non-lethal PCR at 0, 66, 105 dpe (rectal probe) or by PCR and histology (posterior intestine) at 175 dpe. Specific antibodies against E. leei (Ab-Eleei) were immunohistochemically detected on infected intestinal GSB tissues by their immunoreactivity against the parasite. Total serum IgM was detected by means of ELISA with a Pab against GSB IgM. Total serum peroxidases were measured in a plate assay by incubating with 3,3V,5,5V-tetramethylbenzidine hydrochloride. Prevalence of infection in survivor fish was 5.8 % (0 dpe), 7.8 % (66 dpe) and then dropped to 0, whereas in naïve fish it reached 40 % at 105 dpe and then lowered to 22 % at 175 dpe. The percentage of survivor fish with Ab-Eleei was similar throughout the experiment (67-76 %), whereas naïve fish lacked detectable Ab-Eleei until 105 dpe (only 20 % fish had specific antibodies), reaching 67 % at 175 dpe. Similarly, total serum IgM was significantly higher in survivor than in naïve fish at 0 and 175 dpe, but it peaked later in survivor (175 dpe) than in naïve (105 dpe) fish. Peroxidases were significantly higher in naïve fish in all sampling points, except at 175 dpe, when they dropped to the levels found in survivor animals. Therefore, survivor GSB have acquired immunity and resistance to the re-infection, which seems to be related to Ab-Eleei levels, as survivor had higher percentage of Ab-Eleei+ fish with higher IgM levels from the beginning, whereas in naïve fish the acquirement of Ab-Eleei took more than 3 months. By contrast, naïve fish had a higher level of peroxidases, which seemed “switched off” in survivor fish.
DescriptionComunicación presentada en el 9th International Symposium on Fish Parasites, celebrado en Valencia, España, del 31 de agosto al 4 de septiembre de 2015
Appears in Collections:(IATS) Comunicaciones congresos
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