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Title

A Bac transgenic analysis of the asip1 locus reveals developmental mechanisms of dorso-ventral pigmentation in fish.

AuthorsCal, Laura; Gómez-Marín, Carlos ; Gómez-Skarmeta, José Luis ; Cerdá-Reverter, José Miguel ; Kelsh, Robert N.; Rotllant, Josep
KeywordsDorso-ventral gradient
Transgenesis
Zebra sh
Agouti
Pigmentation
Issue Date25-Jun-2015
CitationThe International Symposium on Genetics in Aquaculture XII (2015)
AbstractPigment abnormalities are one of the leading causes in performance loss in at sh aquaculture. Nevertheless, and in spite of the importance of the problem, there is scanty information about the molecular regulation of sh pigmentation which makes the control of this problem di cult. To date, the most important regulator of skin and hair pigmentation in vertebrates is the Agouti signaling protein (ASIP)- melanocortin 1 receptor (MC1R) system: the agouti locus encodes the Agouti-signaling protein (ASIP), which antagonizes the e ects of -Melanocyte- Stimulating hormone (-MSH) binding to the MC1R on the follicular melanocyte, resulting in a decrease in the production of eumelanins (dark pigments) and an increase in pheomelanins (red/yellow pigments). A key aspect of mammalian pigment pattern, that is widely conserved, is the pale ventrum and darker dorsum; the underlying mechanism is regulation of ASIP expression, with high ventral Agouti driving pale belly color. Likewise, sh often show a pale ventrum, but here the pigmentation mechanism is very di erent with independent cell-types, each expressing chemically distinct pigments (not just melanins), being di erentially distributed. Recently, we have shown that not only the agouti gene (asip1), but also even the elevated ventral expression levels, are conserved in sh. It is of interest, in order to know the molecular regulation of sh pigmentation, to establish how Asip1 functions to regulate sh pigment pattern formation. In order to explore the mechanism of Asip1 in zebra sh pigment pat tern formation, we used a BAC transgenic approach to allow characterization of asip1 cis-regulation regions. We have generated a BAC transgenic zebra sh line in which 5 and 3 agouti locus regulatory regions direct eGFP overexpression. Our initial observations showed a graded dorso-ventral expression of eGFP. Therefore, eGFP expression in the asip1- iTol2-eGFP-BAC transgenic sh faithfully recapitulated the endogenous expression pattern of asip1 mRNA. This opens the way to characterizing the mechanisms generating this crucial graded distribution of asip1
DescriptionComunicación presentada en The International Symposium on Genetics in Aquaculture XII, celebrado en Santiago de Compostela, España, del 21 al 27 de junio de 2015
URIhttp://hdl.handle.net/10261/143843
Appears in Collections:(IIM) Comunicaciones congresos
(IPLA) Comunicaciones congresos
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