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Transcriptome profiling of european sea bass brain and adipose tissue responses to photoperiod to identify puberty onset related genes

AutorMartins, R.; Pinto, Pedro M.; Louro, B.; Marques, J. P.; Gómez, Ana ; Zanuy, Silvia ; Carrillo, Manuel ; Canário, Adelino V. M.
Fecha de publicación25-may-2014
Citación10th International Symposium on Reproductive Physiology of Fish (2014)
Resumen[Introduction] Puberty onset is initiated by the activation of the hypothalamus-pituitary-gonadal (HPG) axis to produce gonadotropins and its timing is influenced by internal and external factors, including photoperiod. However, how these external signals are integrated and which gene networks are triggered to initiate this process is still unknown. Thus, the aim of this study was to identify the gene networks that respond to either puberty inhibiting (continuous light-CC) or accelerating (short days- SD) photoperiod regimes at the early events of puberty onset in sea bass, as a step to understand the high incidence of precocious puberty of male sea bass in culture. To achieve this aim, the global transcriptomic changes occurring in pre-pubertal sea bass brain and adipose tissue were characterized throughout the first month after changing photoperiod regimens known to influence puberty onset in sea bass.
[Methods] Immature sea bass of two sizes ¿ large fish, the majority expected to go into puberty, and small fish, not expected to go into puberty - were either maintained in natural photoperiod or changed from natural photoperiod to photoperiod regimens known to delay (continuous light) or accelerate (long to short days) gametogenesis progression. SuperSAGE (Serial Analysis of Gene Expression) libraries (72) were constructed from brain or adipose tissue of fish exposed to the three photoperiod regimens for one to 38 days and sequenced by next generation SOLiD4 sequencing. Transcript tags were extracted, counted and their expression profiles statistically analyzed by pairwise comparisons between photoperiod regimens with false discovery rates < 0.05. The differentially expressed transcripts were mapped to the sea bass genome and available cDNAs using a multi-step Blast approach and mapped to cell pathways for enrichment analysis.
[Results and Discussion] Gene annotation allowed matching 35-54% of the differentially expressed tags to known proteins and 20-24% to anonymous DNAs, with a global annotation rate up to 78%. Genes related to the brain circadian clock, to reproduction, to metabolism and neurotransmitter signaling were identified as differentially expressed between photoperiods in large or small fish in brain and/or adipose tissue.
[Conclusion] In this study we successfully identified key genes and signaling pathways that are changed centrally and peripherally during the early events in response to photoperiod changes highlighting candidate genes and pathways that may be involved in triggering the onset of puberty in sea bass.
DescripciónComunicación presentada en el 10th International Symposium on Reproductive Physiology of Fish, celebrado en Olhao, Portugal, del 25 al 30 de mayo de 2014
URIhttp://hdl.handle.net/10261/143834
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