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Title

Protective role of DNJ-27/Erdj5 in Caenorhabditis elegans models of human neurodegenerative diseases

AuthorsMuñoz-Lobato, Fernando ; Rodríguez-Palero, María Jesús ; Naranjo-Galindo, Francisco José ; Miranda-Vizuete, Antonio
Issue DateJun-2013
CitationVIII Meeting of the Spanish Group of Research on Free Radicals (2013)
AbstractCells have developed quality control systems for protection against proteotoxicity. Misfolded and aggregation-prone proteins, which are behind the initiation and progression of many neurodegenerative diseases, are known to challenge the proteostasis network of the cells. We aimed to explore the role of DNJ-27/ERdj5, an ER-resident protein required as a disulfide reductase for the degradation of misfolded proteins, in well-established Caenorhabditis elegans models of Alzheimer, Parkinson and Huntington diseases. We demonstrate that DNJ-27 is an ER luminal protein and that its expression is induced upon ER stress via IRE-1/XBP-1. When dnj-27 expression is downregulated by RNAi we find an increase in the aggregation and associated pathological phenotypes (paralysis and motility impairment) caused by human β-amyloid peptide, α-synuclein and polyglutamine proteins. In turn, DNJ-27 overexpression ameliorates these deleterious phenotypes. Surprisingly, despite being an ER-resident protein, we show that dnj-27 downregulation alters cytoplasmic protein homeostasis and causes mitochondrial fragmentation. We further demonstrate that DNJ-27 overexpression substantially protects against the mitochondrial fragmentation caused by human β-amyloid and α-synuclein peptides in these worm models. Together, we identify C. elegans dnj-27 as a novel protective gene for the toxicity associated with the expression of human β-amyloid peptide, α-synuclein and polyglutam ine proteins, implying a protective role of ERdj5 in Alzheimer, Parkinson and Huntington diseases. Our data support a scenario where the leve ls of DNJ-27/ERdj5 in the endoplasmic reticulum impact cytoplasmic protein homeosta sis and the integrity of the mitochondrial network which might underlie its protective effect s in models of proteotoxicity associated to human neurodegenerative diseases.
DescriptionTrabajo presentado en la VIII Meeting of the Spanish Group of Research on Free Radicals (GEIRLI), celebradaen Valencia del 3 al 5 de junio de 2013.-- Muñoz-Lobato, Fernando et al.
URIhttp://hdl.handle.net/10261/143752
Appears in Collections:(IBIS) Comunicaciones congresos
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