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Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/143734
Título

TRX-1 Regulates SKN-1 Nuclear Localization Cell Non-autonomously in Caenorhabditis elegans

AutorMcCallum, Katie C.; Liu, Bin; Fierro-González, Juan Carlos; Swoboda, Peter; Arur, Swathi; Miranda-Vizuete, Antonio ; Garsin, Danielle A.
Palabras claveCell non-autonomous signaling
ASJ neurons
Thioredoxin
Oxidative stress response
Caenorhabditis elegans
Fecha de publicación1-may-2016
EditorGenetics Society of America
CitaciónGenetics 203(1): 387-402 (2016)
ResumenThe Caenorhabditis elegans oxidative stress response transcription factor, SKN-1, is essential for the maintenance of redox homeostasis and is a functional ortholog of the Nrf family of transcription factors. The numerous levels of regulation that govern these transcription factors underscore their importance. Here, we add a thioredoxin, encoded by trx-1, to the expansive list of SKN-1 regulators. We report that loss of trx-1 promotes nuclear localization of intestinal SKN-1 in a redox-independent, cell non-autonomous fashion from the ASJ neurons. Furthermore, this regulation is not general to the thioredoxin family, as two other C. elegans thioredoxins TRX-2 and TRX-3 do not play a role in this process. Moreover, TRX-1-dependent regulation requires signaling from the p38 MAPK signaling pathway. However, while TRX-1 regulates SKN-1 nuclear localization, classical SKN-1 transcriptional activity associated with stress response remains largely unaffected. Interestingly, RNA-Seq analysis revealed that loss of trx-1 elicits a general, organism-wide down-regulation of several classes of genes; those encoding for collagens and lipid transport being most prevalent. Together, these results uncover a novel role for a thioredoxin in regulating intestinal SKN-1 nuclear localization in a cell non-autonomous manner, thereby contributing to the understanding of the processes involved in maintaining redox homeostasis throughout an organism.
Versión del editorhttp://doi.org/10.1534/genetics.115.185272
URIhttp://hdl.handle.net/10261/143734
DOI10.1534/genetics.115.185272
Identificadoresissn: 0016-6731
e-issn: 1943-2631
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