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http://hdl.handle.net/10261/143729
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Campo DC | Valor | Lengua/Idioma |
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dc.contributor.author | Buckman, Clayton | - |
dc.contributor.author | Ozanon, Christophe | - |
dc.contributor.author | Qiu, Jing | - |
dc.contributor.author | Sutovsky, Miriam | - |
dc.contributor.author | Carafa, Joyce Ann | - |
dc.contributor.author | Rawe, Vanesa Y. | - |
dc.contributor.author | Manandhar, Gaurishankar | - |
dc.contributor.author | Miranda-Vizuete, Antonio | - |
dc.contributor.author | Sutovsky, Peter | - |
dc.date.accessioned | 2017-02-10T07:54:57Z | - |
dc.date.available | 2017-02-10T07:54:57Z | - |
dc.date.issued | 2013-05-29 | - |
dc.identifier | issn: 1932-6203 | - |
dc.identifier.citation | PLoS ONE 8(5): e61000 (2013) | - |
dc.identifier.uri | http://hdl.handle.net/10261/143729 | - |
dc.description.abstract | Spermatid specific thioredoxin-3 (SPTRX3 or TXNDC8) is a testis/male germ line specific member of thioredoxin family that accumulates in the superfluous cytoplasm of defective human spermatozoa. We hypothesized that semen levels of SPTRX3 are reflective of treatment outcome in assisted reproductive therapy (ART) couples treated by in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI). Relationship between SPTRX3 and treatment outcome was investigated in 239 couples undergoing ART at an infertility clinic. Sperm content of SPTRX3 was evaluated by flow cytometry and epifluorescence microscopy, and correlated with clinical semen analysis parameters, and data on embryo development and pregnancy establishment. High SPTRX3 levels (>15% SPTRX3-positive spermatozoa) were found in 51% of male infertility patients (n¿=¿72), in 20% of men from couples with unexplained, idiopathic infertility (n¿=¿61) and in 14% of men from couples previously diagnosed with female-only infertility (n¿=¿85). Couples with high SPTRX3 produced fewer two-pronuclear zygotes and had a reduced pregnancy rate (19.2% pregnant with >15% SPTRX3-positive spermatozoa vs. 41.2% pregnant with <5% SPTRX3-positive sperm; one-sided p<0.05). The average pregnancy rate of all 239 couples was 25.1%. Live birth rate was 19.2% and lowest average SPTRX3 levels were found in couples that delivered twins. Men with >15% of SPTRX3-positive spermatozoa, a cutoff value established by ROC analysis, had their chance of fathering children by IVF or ICSI reduced by nearly two-thirds. The percentage of SPTRX3-positive spermatozoa had predictive value for pregnancy after ART. Gradient purification and sperm swim-up failed to remove all SPTRX3-positive spermatozoa from semen prepared for ART. In summary, the elevated semen content of SPTRX3 in men from ART couples coincided with reduced incidence of pregnancy by IVF or ICSI, identifying SPTRX3 as a candidate biomarker reflective of ART outcome | - |
dc.description.sponsorship | This study was funded by grant number 1R21HD066333-01A1 from the National Institutes of Health, NICHD, grant number CB000414 from the Research Board of the University of Missouri, seed funding from the Food for The 21st Century Program of the University of Missouri and Undergraduate Summer Research Internship, College of Agriculture, Food and Natural Resources (CAFNR), University of Missouri. | - |
dc.publisher | Public Library of Science | - |
dc.relation.isversionof | Publisher's version | - |
dc.rights | openAccess | - |
dc.title | Semen levels of spermatid-specific thioredoxin-3 correlate with pregnancy rates in ART couples | - |
dc.type | artículo | - |
dc.identifier.doi | 10.1371/journal.pone.0061000 | - |
dc.relation.publisherversion | http://doi.org/10.1371/journal.pone.0061000 | - |
dc.date.updated | 2017-02-10T07:54:58Z | - |
dc.description.version | Peer Reviewed | - |
dc.language.rfc3066 | eng | - |
dc.rights.license | https://creativecommons.org/licenses/by/4.0/ | - |
dc.contributor.funder | Eunice Kennedy Shriver National Institute of Child Health and Human Development (US) | - |
dc.contributor.funder | National Institutes of Health (US) | - |
dc.relation.csic | Sí | - |
dc.identifier.funder | http://dx.doi.org/10.13039/100009633 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/100000002 | es_ES |
dc.identifier.pmid | 23734172 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | With Fulltext | - |
item.cerifentitytype | Publications | - |
item.openairetype | artículo | - |
item.grantfulltext | open | - |
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