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Title

Catabolismo de los polihidroxialcanoatos en la bacteria depredadora “Bdellovibrio Bacteriovorus”: aplicaciones biotecnológicas y diseño de nuevos sistemas para la extracción de bioplástico en cultivos bacterianos

AuthorsMartínez, Virginia
AdvisorPrieto, María Auxiliadora
KeywordsBioplásticos
Sistemas de downstream
Polyhydroxyalcanoatos
Pseudomonas putida
Bacterias depredadoras
Bdellovibrio bacteriovoru
Issue Date2013
PublisherCSIC - Centro de Investigaciones Biológicas Margarita Salas (CIB)
Universidad Complutense de Madrid
AbstractBdellovibrio bacteriovorus HD100 is an obligate predator that invades and grows within the periplasm of Gram-negative bacteria, including mcl-polyhydroxyalkanoate (PHA) producers such as Pseudomonas putida. We investigated the impact of prey PHA content on the predator fitness and the potential advantages for preying on a PHA producer. Using a new procedure to control P. putida KT2442 cell size we demonstrated that the number of Bdellovibrio progeny depends on the prey biomass and not on the viable prey cell number or PHA content. The presence of mcl-PHA hydrolyzed products (monomers, dimers and trimers) in the culture supernatant after predation on P. putida KT42Z, a PHA producing strain lacking PhaZ depolymerase confirmed the ability of Bdellovibrio to degrade the prey´s PHA. Predator motility was significantly higher when growing on PHA accumulating preys. External addition of PHA polymer (latex suspension) to Bdellovibrio preying on the PHA minus mutant P. putida KT42C1 restored predator movement, suggesting that PHA is a key prey component to sustain predator natatory speed. High velocities observed in Bdellovibrio preying on the PHA producing strain were correlated to high intracellular ATP levels of the predator. These effects brought Bdellovibrio fitness benefits as predation on PHA producers was more efficient than predation on non-producing bacteria. Accumulating data also indicated that Bdellovibrio can be potentially used as a novel lytic system for PHA extraction from accumulating bacteria.
Description155 p.-15 fig.-3 anexos. Esta Tesis se presenta bajo el formato de artículos publicados
URIhttp://hdl.handle.net/10261/143723
Appears in Collections:(CIB) Tesis
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