English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/143549
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Sheep experimentally infected with a human isolate of Anaplasma phagocytophilum serve as a host for infection of Ixodes scapularis ticks

AuthorsKocan, Katherine M.; Busby, Ann T.; Coburn, Lisa A.; Galindo, Ruth C. ; Ayllón, Nieves ; Blouin, Edmour F.; Fuente, José de la
KeywordsIxodes scapularis
Anaplasma phagocytophilum
Ticks
Tick-borne pathogen
Sheep model
Issue Date2012
PublisherElsevier
CitationTicks and Tick-Borne Diseases 3(3): 147-153 (2012)
AbstractAnaplasma phagocytophilum, first identified as a pathogen of ruminants in Europe, has more recently been recognized as an emerging tick-borne pathogen of humans in the U.S. and Europe. . A. phagocytophilum is transmitted by . Ixodes spp., but the tick developmental cycle and pathogen/vector interactions have not been fully described. In this research, we report on the experimental infection of sheep with the human NY-18 isolate of . A. phagocytophilum which then served as a host for infection of . I. scapularis nymphs and adults. . A. phagocytophilum was propagated in the human promyelocytic cell line, HL-60, and the infected cell cultures were then used to infect sheep by intravenous inoculation. Infections in sheep were confirmed by PCR and an . Anaplasma-competitive ELISA. Clinical signs were not apparent in any of the infected sheep, and only limited hematologic and mild serum biochemical abnormalities were identified. While . A. phagocytophilum morulae were rarely seen in neutrophils, blood film evaluation revealed prominent large granular lymphocytes, occasional plasma cells, and rare macrophages. Upon necropsy, gross lesions were restricted to the lymphoid system. Mild splenomegaly and lymphadenomegaly with microscopic evidence of lymphoid hyperplasia was observed in all infected sheep. Female . I. scapularis that were allowed to feed and acquire infection on each of the 3 experimentally infected sheep became infected with . A. phagocytophilum as determined by PCR of guts (80-87%) and salivary glands (67-100%). Female . I. scapularis that acquired infection as nymphs on an experimentally infected sheep transmitted . A. phagocytophilum to a susceptible sheep, thus confirming transstadial transmission. Sheep proved to be a good host for the production of . I. scapularis infected with this human isolate of . A. phagocytophilum, which can be used as a model for future studies of the tick/pathogen interface.
URIhttp://hdl.handle.net/10261/143549
DOI10.1016/j.ttbdis.2012.01.004
Identifiersdoi: 10.1016/j.ttbdis.2012.01.004
issn: 1877-959X
e-issn: 1877-9603
Appears in Collections:(IREC) Artículos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.