English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/143516
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar otros formatos: Exportar EndNote (RIS)Exportar bibText (RIS)Exportar csv (RIS)
Título

MHC class II B diversity in blue tits: a preliminary study

Autor Rivero de Aguilar, Juan ; Schut, Elske; Merino, Santiago ; Martínez, Javier ; Komdeur, Jan; Westerdahl, Helena
Palabras clave Cyanistes caeruleus
Parus caeruleus
Passerine
Major histocompatibility complex
Fecha de publicación 2013
EditorJohn Wiley & Sons
European Society of Evolutionary Biology
Society for the Study of Evolution
Citación Ecology and Evolution 3(7): 1878-1889 (2013)
ResumenIn this study, we partly characterize major histocompatibility complex (MHC) class II B in the blue tit (Cyanistes caeruleus). A total of 22 individuals from three different European locations: Spain, The Netherlands, and Sweden were screened for MHC allelic diversity. The MHC genes were investigated using both PCR-based methods and unamplified genomic DNA with restriction fragment length polymorphism (RFLP) and southern blots. A total of 13 different exon 2 sequences were obtained independently from DNA and/or RNA, thus confirming gene transcription and likely functionality of the genes. Nine out of 13 alleles were found in more than one country, and two alleles appeared in all countries. Positive selection was detected in the region coding for the peptide binding region (PBR). A maximum of three alleles per individual was detected by sequencing and the RFLP pattern consisted of 4-7 fragments, indicating a minimum number of 2-4 loci per individual. A phylogenetic analysis, demonstrated that the blue tit sequences are divergent compared to sequences from other passerines resembling a different MHC lineage than those possessed by most passerines studied to date. © 2013 The Authors. Ecology and Evolution.
URI http://hdl.handle.net/10261/143516
Identificadoresdoi: 10.1002/ece3.598
issn: 2045-7758
Aparece en las colecciones: (MNCN) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
EcolEvol 3(7) 1878-1889 (2013).pdf785,75 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.