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Title

EPS-SJ exopolisaccharide produced by the strain Lactobacillus paracasei subsp. paracasei BGSJ2-8 is involved in adhesion to epithelial intestinal cells and decrease on E. coli association to Caco-2 cells

AuthorsZivkovic, Milica; Miljkovic, Marija; Ruas-Madiedo, Patricia ; Markelic, Milica B.; Veljovic, Katarina; Tolinački, Maja; Soković-Bajić, Svetlana; Korac, Aleksandra; Golic, Natasa
KeywordsGALT lymphocytes
EPS-SJ, adhesion
Lactobacillus paracasei
E. coli association
Issue DateMar-2016
PublisherFrontiers Media
CitationFrontiers in Microbiology 7: 286 (2016)
AbstractThe aim of this study was to determine the role of an exopolysaccharide produced by natural dairy isolate Lactobacillus paracasei subsp. paracasei BGSJ2-8, in the adhesion to intestinal epithelial cells and a decrease in Escherichia coli's association with Caco-2 cells. Annotation of the BGSJ2-8 genome showed the presence of a gene cluster, epsSJ, which encodes the biosynthesis of the strain-specific exopolysaccharide EPS-SJ, detected as two fractions (P1 and P2) by size exclusion chromatography (SEC) coupled with multi-angle laser light scattering (MALLS) detection. SEC-MALLS analysis revealed that an EPS-SJ- mutant (EPS7, obtained by insertion mutagenesis of the glps_2198 gene encoding primary glycosyltransferase) does not produce the P2 fraction of EPS-SJ. Transmission electron microscopy showed that EPS7 mutant has a thinner cell wall compared to the EPS-SJ strain BGSJ2-83 (a plasmid free-derivative of BGSJ2-8). Interestingly, strain BGSJ2-83 showed higher adhesion to Caco-2 epithelial intestinal cell line than the EPS7 mutant. Accordingly, BGSJ2-83 effectively reduced E. coli ATCC25922's association with Caco-2 cells, while EPS7 did not show statistically significant differences. In addition, the effect of EPS-SJ on the proliferation of lymphocytes in gastrointestinal associated lymphoid tissue (GALT) was tested and the results showed that the reduction of GALT lymphocyte proliferation was higher by BGSJ2-83 than by the mutant. To the best of our knowledge this is the first report indicating that the presence of EPS (EPS-SJ) on the surface of lactobacilli can improve communication between bacteria and intestinal epithelium*Implying its possible role in gut colonization.
Publisher version (URL)https://doi.org/10.3389/fmicb.2016.00286
URIhttp://hdl.handle.net/10261/143310
DOIhttp://dx.doi.org/10.3389/fmicb.2016.00286
Identifiersissn: 1664-302X
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