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'Artilysation' of endolysin λSa2lys strongly improves its enzymatic and antibacterial activity against streptococci

AuthorsRodríguez, Lorena CSIC ORCID; Gutiérrez, Diana CSIC ORCID; Martínez Fernández, Beatriz CSIC ORCID ; Rodríguez González, Ana CSIC ORCID; García Suárez, María Pilar CSIC ORCID
Issue Date26-Oct-2016
PublisherNature Publishing Group
CitationScientific Reports 6: 35382 (2016)
AbstractEndolysins constitute a promising class of antibacterials against Gram-positive bacteria. Recently, endolysins have been engineered with selected peptides to obtain a new generation of lytic proteins, Artilysins, with specific activity against Gram-negative bacteria. Here, we demonstrate that artilysation can also be used to enhance the antibacterial activity of endolysins against Gram-positive bacteria and to reduce the dependence on external conditions. Art-240, a chimeric protein of the anti-streptococcal endolysin λSa2lys and the polycationic peptide PCNP, shows a similar species specificity as the parental endolysin, but the bactericidal activity against streptococci increases and is less affected by elevated NaCl concentrations and pH variations. Time-kill experiments and time-lapse microscopy demonstrate that the killing rate of Art-240 is approximately two-fold higher compared to wildtype endolysin λSa2lys, with a reduction in viable bacteria of 3 log units after 10 min. In addition, lower doses of Art-240 are required to achieve the same bactericidal effect.
Publisher version (URL)https://doi.org/10.1038/srep35382
Identifiersissn: 2045-2322
Appears in Collections:(IPLA) Artículos
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