English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/143241
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:


A proteomic approach towards understanding the cross talk between Bacteroides fragilis and Bifidobacterium longum in coculture

AuthorsRios-Covián, David CSIC ORCID; Sánchez García, Borja CSIC ORCID; Martínez Álvarez, Noelia CSIC ORCID CVN; Cuesta Suárez, Isabel CSIC; Hernández-Barranco, Ana María CSIC ORCID; González de los Reyes-Gavilán, Clara CSIC ORCID ; Gueimonde Fernández, Miguel CSIC ORCID
KeywordsCross talk
Issue Date4-Mar-2016
PublisherCanadian Science Publishing
CitationCanadian Journal of Microbiology 62: 623-628 (2016)
AbstractA better understanding of the interactions among intestinal microbes is needed to decipher the complex cross talk that takes place within the human gut. Bacteroides and Bifidobacterium genera are among the most relevant intestinal bacteria, and it has been previously reported that coculturing of these 2 microorganisms affects their survival. Therefore, coculturing of Bifidobacterium longum NB667 and Bacteroides fragilis DSMZ2151 was performed with the aim of unravelling the mechanisms involved in their interaction. To this end, we applied proteomic (2D-DIGE) analyses, and by chromatographic techniques we quantified the bacterial metabolites produced during coincubation. Coculture stimulated the growth of B. longum, retarding that of B. fragilis, with concomitant changes in the production of some proteins and metabolites of both bacteria. The combined culture promoted upregulation of the bifidobacterial pyruvate kinase and downregulation of the Bacteroides phosphoenolpyruvate carboxykinase - 2 enzymes involved in the catabolism of carbohydrates. Moreover, B. fragilis FKBP-type peptidyl-prolyl cis-trans isomerase, a protein with chaperone-like activity, was found to be overproduced in coculture, suggesting the induction of a stress response in this microorganism. This study provides mechanistic data to deepen our understanding of the interaction between Bacteroides and Bifidobacterium intestinal populations. Copyright NRC Research Press
Publisher version (URL)https://doi.org/10.1139/cjm-2015-0804
Identifiersissn: 1480-3275
Appears in Collections:(IPLA) Artículos
Files in This Item:
File Description SizeFormat 
proteomic approach-RiosCovian.pdf655,82 kBAdobe PDFThumbnail
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.