Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/143102
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Title

Immunization with LytB protein of Streptococcus pneumoniae activates complement-mediated phagocytosis and induces protection against pneumonia and sepsis

AuthorsCorsini, B.; Aguinagalde, Leire CSIC ORCID; Ruiz, Susana; Domenech, Mirian CSIC ORCID; Antequera, Marisa; Fenoll, Asunción; García, Pedro CSIC ORCID ; García, Ernesto CSIC ORCID ; Yuste, José CSIC ORCID
KeywordsStreptococcus pneumoniae,
Cell wall hydrolases
LytB
Vaccine protein
Phagocytosis
Complement immunity
Issue Date7-Dec-2016
PublisherElsevier
CitationVaccine 34(50):6148-6157 (2016)
AbstractThe cell wall glucosaminidase LytB of Streptococcus pneumoniae is a surface exposed protein involved in daughter cell separation, biofilm formation and contributes to different aspects of the pathogenesis process. In this study we have characterized the antibody responses after immunization of mice with LytB in the presence of alhydrogel as an adjuvant. Enzyme-linked immunosorbent assays measuring different subclasses of immunoglobulin G, demonstrated that the antibody responses to LytB were predominantly IgG1 and IgG2b, followed by IgG3 and IgG2a subclasses. Complement-mediated immunity against two different pneumococcal serotypes was investigated using sera from immunized mice. Immunization with LytB increased the recognition of S. pneumoniae by complement components C1q and C3b demonstrating that anti-LytB antibodies trigger activation of the classical pathway. Phagocytosis assays showed that serum containing antibodies to LytB stimulates neutrophil-mediated phagocytosis against S. pneumoniae. Animal models of infection including invasive pneumonia and sepsis were performed with two different clinical isolates. Vaccination with LytB increased bacterial clearance and induced protection demonstrating that LytB might be a good candidate to be considered in a future protein-based vaccine against S. pneumoniae.
Description34 p.-8 fig.
Publisher version (URL)http://dx.doi.org/10.1016/j.vaccine.2016.11.001
URIhttp://hdl.handle.net/10261/143102
DOI10.1016/j.vaccine.2016.11.001
ISSN0264-410X
Appears in Collections:(CIB) Artículos

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