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Implementation of the agmatine-controlled expression system for inducible gene expression in Lactococcus lactis

AutorLinares, Daniel M. ; Álvarez Sieiro, Patricia ; Río Lagar, Beatriz del ; Ladero Losada, Víctor Manuel ; Redruello, Begoña ; Martín, M. Cruz ; Fernández García, María ; Álvarez González, Miguel Ángel
Palabras claveLactococcus lactis
Myxococcus xanthus
Agmatine induction
Expression vector
Prolyl-endopeptidase
Fecha de publicación30-dic-2015
EditorBioMed Central
CitaciónMicrobial Cell Factories 14: 208 (2015)
Resumen[Background] Lactococcus lactis has been safely consumed in fermented foods for millennia. This Gram-positive bacterium has now become of industrial importance as an expression host for the overproduction of lipopolysaccharide-free recombinant proteins used as food ingredients, therapeutic proteins and biotechnological enzymes.
[Results] This paper reports an agmatine-controlled expression (ACE) system for L. lactis, comprising the lactococcal agmatine-sensor/transcriptional activator AguR and its target promoter P aguB . The usefulness and efficiency of this system was checked via the reporter gene gfp and by producing PEP (Myxococcus xanthus prolyl-endopeptidase), an enzyme of biomedical interest able to degrade the immunotoxic peptides produced during the gastrointestinal breakdown of gluten.
[Conclusion] The ACE system developed in this work was suitable for the efficient expression of the functional recombinant proteins GFP and PEP. The expression system was tightly regulated by the agmatine concentration and allowed high protein production without leakiness.
Versión del editorhttps://doi.org/10.1186/s12934-015-0399-x
URIhttp://hdl.handle.net/10261/143003
DOI10.1186/s12934-015-0399-x
Identificadoresissn: 1475-2859
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