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Título

Middle East respiratory syndrome coronavirus neutralising serum antibodies in dromedary camels: a comparative serological study

AutorReusken, Chantal B. E. M.; Gortázar, Christian
Palabras claveDeutsche Forschungsgemeinschaft
European Union
European Centre For Disease Prevention and Control
Fecha de publicación2013
EditorElsevier
CitaciónLancet Infectious Diseases 13(10): 859-866 (2013)
Resumen[Background]: A new betacoronavirus-Middle East respiratory syndrome coronavirus (MERS-CoV)-has been identified in patients with severe acute respiratory infection. Although related viruses infect bats, molecular clock analyses have been unable to identify direct ancestors of MERS-CoV. Anecdotal exposure histories suggest that patients had been in contact with dromedary camels or goats. We investigated possible animal reservoirs of MERS-CoV by assessing specific serum antibodies in livestock. [Methods]: We took sera from animals in the Middle East (Oman) and from elsewhere (Spain, Netherlands, Chile). Cattle (n=80), sheep (n=40), goats (n=40), dromedary camels (n=155), and various other camelid species (n=34) were tested for specific serum IgG by protein microarray using the receptor-binding S1 subunits of spike proteins of MERS-CoV, severe acute respiratory syndrome coronavirus, and human coronavirus OC43. Results were confirmed by virus neutralisation tests for MERS-CoV and bovine coronavirus. [Findings]: 50 of 50 (100%) sera from Omani camels and 15 of 105 (14%) from Spanish camels had protein-specific antibodies against MERS-CoV spike. Sera from European sheep, goats, cattle, and other camelids had no such antibodies. MERS-CoV neutralising antibody titres varied between 1/320 and 1/2560 for the Omani camel sera and between 1/20 and 1/320 for the Spanish camel sera. There was no evidence for cross-neutralisation by bovine coronavirus antibodies. [Interpretation]: MERS-CoV or a related virus has infected camel populations. Both titres and seroprevalences in sera from different locations in Oman suggest widespread infection.
URIhttp://hdl.handle.net/10261/142869
DOI10.1016/S1473-3099(13)70164-6
Identificadoresdoi: 10.1016/S1473-3099(13)70164-6
issn: 1473-3099
e-issn: 1474-4457
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