English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/1428
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

Molecular characterization of Anaplasma platys strains from dogs in Sicily, Italy

AutorFuente, José de la ; Torina, Alessandra; Naranjo, María Victoria ; Nicosia, Silviane; Alongi, Angelina; La Mantia, Francesco; Kocan, Katherine M.
Fecha de publicación26-jul-2006
EditorBioMed Central
CitaciónBMC Veterinary Research 2006, 2:24
Resumen[Background] The genetic diversity of Anaplasma platys (Rickettsiales: Anaplasmataceae) strains is currently poorly defined. The present study was designed to characterize A. patys strains in dogs from Palermo, Sicily, Italy, using a combination of PCR and sequence analysis of the 16S rDNA, heat shock operon groESL and citrate synthase (gltA) genes.
[Results] Blood was collected from 344 dogs (111 pet dogs, 122 pound dogs and 111 hunting dogs) during 2003–2005 in the Province of Palermo, Sicily, Italy. The prevalence of A. platys in dogs in Sicily, as demonstrated by PCR and sequence analysis of the 16S rDNA, groESL and gltA genes, was 4%. None of the samples were positive for A. marginale, A. centrale, A. ovis and A. phagocytophilum DNA. Three different gltA genotypes of A. platys were identified in dogs from Sicily. Two of the gltA sequences of Sicilian A. platys strains were different from sequences reported previously. However, one of the gltA, 16S rDNA and groESL sequences were identical to the sequence of A. platys strains from other regions of the world characterized previously.
[Conclusion] At least three different strains of A. platys were identified in dogs from Sicily by PCR and sequence analyses of the 16S rDNA, groESL and gltA genes. The results reported herein suggested that genetic diversity of A. platys strains may be similar to A. ovis, but lower than the diversity reported for A. marginale and A. phagocytophilum. This lower genetic diversity may have resulted from restricted movement of infected hosts compared to A. marginale-infected cattle and/or the limited host range of A. ovis and A. platys as compared with A. phagocytophilum. These results expand our knowledge about A. platys and encourage further research for analysis of the genetic variation of A. platys strains worldwide.
Versión del editorhttp://dx.doi.org/10.1186/1746-6148-2-24
URIhttp://hdl.handle.net/10261/1428
DOI10.1186/1746-6148-2-24
ISSN1746-6148
Aparece en las colecciones: (IREC) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
1746-6148-2-24.pdf255,52 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.