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Title

Reconstitution of respiratory complex I on a biomimetic membrane supported on gold electrodes

AuthorsGutiérrez-Sanz, Óscar ; Olea, David ; Pita, Marcos ; Batista, Ana P.; Alonso, Álvaro; Pereira, Manuela M.; Vélez, Marisela ; Lacey, Antonio L. de
KeywordsInfrared-absorption spectroscopy
Nadh-Ubiquinone oxidoreductase
Escherichia-coli
Rhodothermus-marinus
Lipid-membrane
Cluster N2
Electrochemistry
Oxidation
Transport
Bilayer
Adenosinetriphosphate
Atomic force microscopy
Biomimetics
Cell membranes
Electrodes
Gold
Organic polymers
Self assembled monolayers
Electrochemical measurements
Electrochemical potential
Membrane-bound enzymes
Mitochondrial membranes
Proton translocation
Respiratory complex
Structure and activities
Thiol self-assembled monolayers
Gold compounds
Artificial membrane
Bacterial protein
Biomimetic material
Proton
Atomic force microscopy
Reduced nicotinamide adenine dinucleotide dehydrogenase (ubiquinone)
Thiol reagent
Chemistry
Electrode
Electron transport
Enzymology
Isolation and purification
Rhodothermus
Bacterial Proteins
Biomimetic Materials
Electrodes
Electron Transport
Electron Transport Complex I
Membranes, Artificial
Microscopy, Atomic Force
Protons
Rhodothermus
Sulfhydryl Reagents
Issue Date2014
PublisherAmerican Chemical Society
CitationLangmuir : the ACS journal of surfaces and colloids 30(29): 9007-9015 (2014)
AbstractFor the first time, respiratory complex I has been reconstituted on an electrode preserving its structure and activity. Respiratory complex I is a membrane-bound enzyme that has an essential function in cellular energy production. It couples NADH:quinone oxidoreduction to translocation of ions across the cellular (in prokaryotes) or mitochondrial membranes. Therefore, complex I contributes to the establishment and maintenance of the transmembrane difference of electrochemical potential required for adenosine triphosphate synthesis, transport, and motility. Our new strategy has been applied for reconstituting the bacterial complex I from Rhodothermus marinus onto a biomimetic membrane supported on gold electrodes modified with a thiol self-assembled monolayer (SAM). Atomic force microscopy and faradaic impedance measurements give evidence of the biomimetic construction, whereas electrochemical measurements show its functionality. Both electron transfer and proton translocation by respiratory complex I were monitored, simulating in vivo conditions. © 2014 American Chemical Society.
Publisher version (URL)http://doi.org/10.1021/la501825r
URIhttp://hdl.handle.net/10261/142779
DOI10.1021/la501825r
Identifiersdoi: 10.1021/la501825r
issn: 0743-7463
e-issn: 1520-5827
Appears in Collections:(ICP) Artículos
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