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Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/142749
Título

Fertility of cryopreserved ovine semen is determined by sperm velocity

AutorOlmo, Enrique del ; Bisbal, Alfonso ; Maroto-Morales, Alejandro ; García-Álvarez, Olga; Ramón, Manuel; Jiménez-Rabadán, Pilar; Martínez-Pastor, Felipe ; Soler, Ana J. ; Garde, José Julián ; Fernández-Santos, M. Rocío
Palabras claveFertility
Cryopreserved semen
Ram
Flow cytometry
Fecha de publicación2013
EditorElsevier
CitaciónAnimal Reproduction Science 138(1-2): 102-109 (2013)
ResumenThe present study aims to examine the predictive value of some sperm parameters on male fertility. Semen samples from six Manchega rams were collected and cryopreserved. Sperm quality was assessed after thawing and after 2 h of incubation, either in the freezing extender (37 °C) or after dilution in Synthetic Oviductal Fluid (SOF) (38 °C, 5% CO2), attempting to mimic the physiological conditions of the female reproductive tract. The following sperm parameters were evaluated: motility and kinetic parameters by computer-assisted semen analyzer (CASA), and sperm viability (propidium iodide), mitochondrial membrane potential (JC-1), apoptotic-like membrane changes (YO-PRO-1), acrosomal status (PNA-FITC), and intracellular calcium (fluo-3) by flow cytometry. Results showed no significant differences between incubation media neither after thawing nor after incubation. There were no significant correlations between fertility and sperm parameters assessed by flow cytometry. However, after incubation in the freezing extender, sperm samples from males with poor fertility yielded less linearity and velocity (P < 0.05) as indicated by motility parameters analyzed by CASA. These results indicate that kinematic sperm motility parameters evaluation by CASA might be useful to identify samples with poor fertility.
Versión del editorhttp://dx.doi.org/10.1016/j.anireprosci.2013.02.007
URIhttp://hdl.handle.net/10261/142749
DOI10.1016/j.anireprosci.2013.02.007
ISSN0378-4320
E-ISSN1873-2232
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